Experiment: MoLS4 with L-Methionine (1mM) as nutrient
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU3308 and DVU3309 are separated by 31 nucleotides DVU3309 and deaD overlap by 13 nucleotides deaD and DVU3311 are separated by 186 nucleotides DVU3311 and DVU3312 are separated by 136 nucleotides DVU3312 and DVU3313 are separated by 30 nucleotides
DVU3308: DVU3308 - metallo-beta-lactamase family protein (TIGR), at 3,485,523 to 3,486,242
DVU3308
DVU3309: DVU3309 - endo/excinuclease amino terminal domain protein (TIGR), at 3,486,274 to 3,486,606
DVU3309
DVU3310: deaD - ATP-dependent RNA helicase, DEAD/DEAH family (TIGR), at 3,486,594 to 3,488,192
deaD
DVU3311: DVU3311 - hypothetical protein (TIGR), at 3,488,379 to 3,488,555
DVU3311
DVU3312: DVU3312 - conserved hypothetical protein (TIGR), at 3,488,692 to 3,489,147
DVU3312
DVU3313: DVU3313 - transcriptional regulator, LysR family (TIGR), at 3,489,178 to 3,490,068
DVU3313
Position (kb)
3486
3487
3488
3489 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3485.664 kb on - strand, within DVU3308 at 3485.711 kb on + strand, within DVU3308 at 3485.730 kb on + strand, within DVU3308 at 3485.735 kb on + strand, within DVU3308 at 3486.031 kb on + strand, within DVU3308 at 3486.073 kb on - strand, within DVU3308 at 3486.098 kb on - strand, within DVU3308 at 3486.108 kb on - strand, within DVU3308 at 3486.120 kb on + strand, within DVU3308 at 3486.178 kb on - strand at 3486.190 kb on + strand at 3486.228 kb on + strand at 3486.251 kb on - strand at 3486.269 kb on - strand at 3486.334 kb on + strand, within DVU3309 at 3486.334 kb on + strand, within DVU3309 at 3486.339 kb on + strand, within DVU3309 at 3486.347 kb on - strand, within DVU3309 at 3486.390 kb on + strand, within DVU3309 at 3486.448 kb on - strand, within DVU3309 at 3486.448 kb on - strand, within DVU3309 at 3486.572 kb on - strand, within DVU3309 at 3486.618 kb on + strand at 3486.641 kb on + strand at 3486.641 kb on + strand at 3486.641 kb on + strand at 3486.649 kb on - strand at 3486.665 kb on - strand at 3486.684 kb on + strand at 3486.718 kb on - strand at 3486.733 kb on + strand at 3486.782 kb on + strand, within deaD at 3486.784 kb on - strand, within deaD at 3486.813 kb on + strand, within deaD at 3486.813 kb on + strand, within deaD at 3486.813 kb on + strand, within deaD at 3486.820 kb on + strand, within deaD at 3486.821 kb on - strand, within deaD at 3486.835 kb on - strand, within deaD at 3486.923 kb on - strand, within deaD at 3486.946 kb on + strand, within deaD at 3486.946 kb on + strand, within deaD at 3487.261 kb on + strand, within deaD at 3487.429 kb on + strand, within deaD at 3487.452 kb on - strand, within deaD at 3487.457 kb on - strand, within deaD at 3487.515 kb on - strand, within deaD at 3487.845 kb on - strand, within deaD at 3487.922 kb on + strand, within deaD at 3488.172 kb on + strand at 3488.172 kb on + strand at 3488.180 kb on - strand at 3488.180 kb on - strand at 3488.261 kb on + strand at 3488.261 kb on + strand at 3488.409 kb on - strand, within DVU3311 at 3488.478 kb on - strand, within DVU3311 at 3488.534 kb on - strand, within DVU3311 at 3488.535 kb on - strand, within DVU3311 at 3488.546 kb on - strand at 3488.558 kb on - strand at 3488.568 kb on + strand at 3488.571 kb on - strand at 3488.608 kb on - strand at 3488.618 kb on + strand at 3488.618 kb on + strand at 3488.626 kb on - strand at 3488.675 kb on + strand at 3488.675 kb on + strand at 3488.870 kb on + strand, within DVU3312 at 3488.876 kb on + strand, within DVU3312 at 3488.971 kb on - strand, within DVU3312 at 3488.972 kb on + strand, within DVU3312 at 3489.011 kb on + strand, within DVU3312 at 3489.019 kb on - strand, within DVU3312 at 3489.039 kb on + strand, within DVU3312 at 3489.047 kb on - strand, within DVU3312 at 3489.059 kb on - strand, within DVU3312 at 3489.119 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with L-Methionine (1mM) as nutrient remove 3,485,664 - DVU3308 0.20 +0.4 3,485,711 + DVU3308 0.26 -0.3 3,485,730 + DVU3308 0.29 +0.5 3,485,735 + DVU3308 0.29 +0.3 3,486,031 + DVU3308 0.71 -0.3 3,486,073 - DVU3308 0.76 -0.7 3,486,098 - DVU3308 0.80 -0.0 3,486,108 - DVU3308 0.81 +1.0 3,486,120 + DVU3308 0.83 -0.1 3,486,178 - -1.8 3,486,190 + +0.2 3,486,228 + +0.6 3,486,251 - -0.2 3,486,269 - +0.3 3,486,334 + DVU3309 0.18 -0.0 3,486,334 + DVU3309 0.18 +0.9 3,486,339 + DVU3309 0.20 -1.3 3,486,347 - DVU3309 0.22 -0.7 3,486,390 + DVU3309 0.35 +0.3 3,486,448 - DVU3309 0.52 -0.4 3,486,448 - DVU3309 0.52 -0.5 3,486,572 - DVU3309 0.89 -1.3 3,486,618 + -1.9 3,486,641 + -1.2 3,486,641 + -1.3 3,486,641 + -1.9 3,486,649 - -1.4 3,486,665 - -2.4 3,486,684 + -1.1 3,486,718 - -2.3 3,486,733 + -0.6 3,486,782 + deaD DVU3310 0.12 -1.2 3,486,784 - deaD DVU3310 0.12 -0.2 3,486,813 + deaD DVU3310 0.14 -1.8 3,486,813 + deaD DVU3310 0.14 -0.6 3,486,813 + deaD DVU3310 0.14 -2.4 3,486,820 + deaD DVU3310 0.14 -1.7 3,486,821 - deaD DVU3310 0.14 -4.3 3,486,835 - deaD DVU3310 0.15 -0.6 3,486,923 - deaD DVU3310 0.21 -2.0 3,486,946 + deaD DVU3310 0.22 -1.6 3,486,946 + deaD DVU3310 0.22 -2.4 3,487,261 + deaD DVU3310 0.42 -0.8 3,487,429 + deaD DVU3310 0.52 -1.9 3,487,452 - deaD DVU3310 0.54 -1.4 3,487,457 - deaD DVU3310 0.54 -3.9 3,487,515 - deaD DVU3310 0.58 -2.2 3,487,845 - deaD DVU3310 0.78 -1.8 3,487,922 + deaD DVU3310 0.83 -1.3 3,488,172 + -1.2 3,488,172 + -1.5 3,488,180 - -0.0 3,488,180 - -1.4 3,488,261 + +0.7 3,488,261 + -0.9 3,488,409 - DVU3311 0.17 -0.8 3,488,478 - DVU3311 0.56 +0.2 3,488,534 - DVU3311 0.88 +0.1 3,488,535 - DVU3311 0.88 +0.1 3,488,546 - -2.0 3,488,558 - -1.2 3,488,568 + +1.4 3,488,571 - +0.4 3,488,608 - +0.2 3,488,618 + +0.0 3,488,618 + +0.8 3,488,626 - -0.3 3,488,675 + -0.4 3,488,675 + +0.2 3,488,870 + DVU3312 0.39 +0.5 3,488,876 + DVU3312 0.40 +1.0 3,488,971 - DVU3312 0.61 -1.6 3,488,972 + DVU3312 0.61 -0.7 3,489,011 + DVU3312 0.70 -1.0 3,489,019 - DVU3312 0.72 +0.4 3,489,039 + DVU3312 0.76 -1.0 3,489,047 - DVU3312 0.78 +0.8 3,489,059 - DVU3312 0.80 +0.5 3,489,119 + -1.2
Or see this region's nucleotide sequence