Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3666c

Experiment: 7H9 with Clofazimine 0.45 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3664c and Rv3665c are separated by 55 nucleotidesRv3665c and Rv3666c are separated by 1 nucleotidesRv3666c and Rv3667 are separated by 707 nucleotides Rv3664c: Rv3664c - Probable dipeptide-transport integral membrane protein ABC transporter DppC, at 4,103,675 to 4,104,475 Rv3664c Rv3665c: Rv3665c - Probable dipeptide-transport integral membrane protein ABC transporter DppB, at 4,104,531 to 4,105,457 Rv3665c Rv3666c: Rv3666c - Probable periplasmic dipeptide-binding lipoprotein DppA, at 4,105,459 to 4,107,084 Rv3666c Rv3667: Rv3667 - Acetyl-coenzyme A synthetase Acs (acetate--CoA ligase) (acetyl-CoA synthetase) (acetyl-CoA synthase) (acyl-activating enzyme) (acetate thiokinase) (acetyl-activating enzyme) (acetate--coenzyme A ligase) (acetyl-coenzyme A synthase), at 4,107,792 to 4,109,747 Rv3667 Position (kb) 4105 4106 4107 4108Strain fitness (log2 ratio) -1 0 1 2at 4104.482 kb on - strandat 4104.482 kb on - strandat 4105.447 kb on + strandat 4105.998 kb on - strand, within Rv3666cat 4106.337 kb on - strand, within Rv3666cat 4106.362 kb on - strand, within Rv3666cat 4106.528 kb on + strand, within Rv3666cat 4106.730 kb on - strand, within Rv3666cat 4106.876 kb on + strand, within Rv3666cat 4107.438 kb on + strandat 4107.439 kb on - strandat 4107.530 kb on + strandat 4107.667 kb on + strandat 4107.695 kb on + strandat 4107.765 kb on + strandat 4107.765 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Clofazimine 0.45 ug/mL
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4,104,482 - -0.3
4,104,482 - -0.5
4,105,447 + -0.6
4,105,998 - Rv3666c 0.33 +0.5
4,106,337 - Rv3666c 0.54 -0.5
4,106,362 - Rv3666c 0.56 +1.8
4,106,528 + Rv3666c 0.66 -0.2
4,106,730 - Rv3666c 0.78 +0.6
4,106,876 + Rv3666c 0.87 +1.6
4,107,438 + +0.1
4,107,439 - -0.4
4,107,530 + -0.5
4,107,667 + -0.1
4,107,695 + -0.1
4,107,765 + +1.8
4,107,765 + +0.6

Or see this region's nucleotide sequence