Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3303c

Experiment: 7H9 with Clofazimine 0.45 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3302c and Rv3303c are separated by 14 nucleotidesRv3303c and Rv3304 are separated by 202 nucleotidesRv3304 and Rv3305c are separated by 18 nucleotides Rv3302c: Rv3302c - Probable glycerol-3-phosphate dehydrogenase GlpD2, at 3,687,685 to 3,689,442 Rv3302c Rv3303c: Rv3303c - NAD(P)H quinone reductase LpdA, at 3,689,457 to 3,690,938 Rv3303c Rv3304: Rv3304 - Conserved protein, at 3,691,141 to 3,691,620 Rv3304 Rv3305c: Rv3305c - Possible N-acyl-L-amino acid amidohydrolase AmiA1 (N-acyl-L-amino acid aminohydrolase), at 3,691,639 to 3,692,808 Rv3305c Position (kb) 3689 3690 3691Strain fitness (log2 ratio) -2 -1 0 1at 3689.493 kb on + strandat 3689.494 kb on - strandat 3690.047 kb on - strand, within Rv3303cat 3690.047 kb on - strand, within Rv3303cat 3690.413 kb on - strand, within Rv3303cat 3690.413 kb on - strand, within Rv3303cat 3690.946 kb on - strandat 3690.950 kb on + strandat 3690.951 kb on - strandat 3690.951 kb on - strandat 3690.951 kb on - strandat 3690.951 kb on - strandat 3691.107 kb on - strandat 3691.160 kb on - strandat 3691.334 kb on - strand, within Rv3304at 3691.468 kb on + strand, within Rv3304at 3691.515 kb on - strand, within Rv3304at 3691.566 kb on - strand, within Rv3304at 3691.566 kb on - strand, within Rv3304at 3691.612 kb on - strandat 3691.618 kb on + strandat 3691.618 kb on + strandat 3691.641 kb on - strandat 3691.860 kb on + strand, within Rv3305c

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Clofazimine 0.45 ug/mL
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3,689,493 + -0.5
3,689,494 - +0.2
3,690,047 - Rv3303c 0.40 -0.4
3,690,047 - Rv3303c 0.40 -2.4
3,690,413 - Rv3303c 0.65 -0.2
3,690,413 - Rv3303c 0.65 -1.8
3,690,946 - +0.5
3,690,950 + +0.3
3,690,951 - -1.2
3,690,951 - -0.6
3,690,951 - -0.7
3,690,951 - -0.2
3,691,107 - -0.8
3,691,160 - -0.8
3,691,334 - Rv3304 0.40 -1.4
3,691,468 + Rv3304 0.68 -0.7
3,691,515 - Rv3304 0.78 -1.8
3,691,566 - Rv3304 0.89 -0.2
3,691,566 - Rv3304 0.89 +0.3
3,691,612 - +1.6
3,691,618 + +0.4
3,691,618 + -0.4
3,691,641 - -0.4
3,691,860 + Rv3305c 0.19 +0.5

Or see this region's nucleotide sequence