Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2728c

Experiment: 7H9 with Clofazimine 0.45 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2726c and Rv2727c are separated by 24 nucleotidesRv2727c and Rv2728c overlap by 4 nucleotidesRv2728c and Rv2729c are separated by 108 nucleotides Rv2726c: Rv2726c - Probable diaminopimelate epimerase DapF (DAP epimerase), at 3,038,931 to 3,039,800 Rv2726c Rv2727c: Rv2727c - Probable tRNA delta(2)-isopentenylpyrophosphate transferase MiaA (IPP transferase) (isopentenyl-diphosphate:tRNA isopentenyltransferase) (iptase) (IPPT), at 3,039,825 to 3,040,769 Rv2727c Rv2728c: Rv2728c - Conserved alanine rich protein, at 3,040,766 to 3,041,461 Rv2728c Rv2729c: Rv2729c - Probable conserved integral membrane alanine valine and leucine rich protein, at 3,041,570 to 3,042,475 Rv2729c Position (kb) 3040 3041 3042Strain fitness (log2 ratio) -2 -1 0 1at 3040.819 kb on - strandat 3040.981 kb on - strand, within Rv2728cat 3041.031 kb on - strand, within Rv2728cat 3041.177 kb on - strand, within Rv2728cat 3041.196 kb on - strand, within Rv2728cat 3041.196 kb on - strand, within Rv2728cat 3041.473 kb on - strandat 3041.473 kb on - strandat 3041.473 kb on - strandat 3041.651 kb on + strandat 3041.651 kb on + strandat 3041.865 kb on + strand, within Rv2729cat 3041.865 kb on + strand, within Rv2729cat 3041.866 kb on - strand, within Rv2729cat 3041.943 kb on - strand, within Rv2729cat 3042.085 kb on + strand, within Rv2729cat 3042.366 kb on + strand, within Rv2729cat 3042.367 kb on - strand, within Rv2729cat 3042.367 kb on - strand, within Rv2729cat 3042.367 kb on - strand, within Rv2729cat 3042.411 kb on + strandat 3042.412 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Clofazimine 0.45 ug/mL
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3,040,819 - -0.9
3,040,981 - Rv2728c 0.31 -1.3
3,041,031 - Rv2728c 0.38 -0.8
3,041,177 - Rv2728c 0.59 +0.2
3,041,196 - Rv2728c 0.62 -0.5
3,041,196 - Rv2728c 0.62 -1.2
3,041,473 - -0.6
3,041,473 - -0.2
3,041,473 - -0.6
3,041,651 + -1.8
3,041,651 + +0.1
3,041,865 + Rv2729c 0.33 +0.1
3,041,865 + Rv2729c 0.33 +0.1
3,041,866 - Rv2729c 0.33 +0.2
3,041,943 - Rv2729c 0.41 +0.8
3,042,085 + Rv2729c 0.57 -2.3
3,042,366 + Rv2729c 0.88 -1.1
3,042,367 - Rv2729c 0.88 -1.6
3,042,367 - Rv2729c 0.88 +0.4
3,042,367 - Rv2729c 0.88 -0.4
3,042,411 + -0.6
3,042,412 - +0.1

Or see this region's nucleotide sequence