Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2242

Experiment: 7H9 with Clofazimine 0.45 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2241 and Rv2242 are separated by 59 nucleotidesRv2242 and Rv2243 are separated by 238 nucleotides Rv2241: Rv2241 - Pyruvate dehydrogenase E1 component AceE (pyruvate decarboxylase) (pyruvate dehydrogenase) (pyruvic dehydrogenase), at 2,512,539 to 2,515,244 Rv2241 Rv2242: Rv2242 - Conserved hypothetical protein, at 2,515,304 to 2,516,548 Rv2242 Rv2243: Rv2243 - Malonyl CoA-acyl carrier protein transacylase FabD (malonyl CoA:ACPM acyltransferase) (MCT), at 2,516,787 to 2,517,695 Rv2243 Position (kb) 2515 2516 2517Strain fitness (log2 ratio) -2 -1 0 1at 2514.348 kb on + strand, within Rv2241at 2515.242 kb on + strandat 2516.532 kb on - strandat 2516.532 kb on - strandat 2516.621 kb on + strandat 2516.690 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Clofazimine 0.45 ug/mL
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2,514,348 + Rv2241 0.67 -1.8
2,515,242 + -1.3
2,516,532 - -1.5
2,516,532 - +0.9
2,516,621 + -0.5
2,516,690 + -1.0

Or see this region's nucleotide sequence