Experiment: MoLS4 with Casaminos as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gpmA and DVU1620 are separated by 4 nucleotides DVU1620 and DVU1621 are separated by 101 nucleotides DVU1621 and DORF12360 are separated by 145 nucleotides DORF12360 and purQ are separated by 52 nucleotides
DVU1619: gpmA - phosphoglycerate mutase, 2,3-bisphosphoglycerate-independent (TIGR), at 1,703,251 to 1,704,786
gpmA
DVU1620: DVU1620 - hypothetical protein (TIGR), at 1,704,791 to 1,705,033
DVU1620
DVU1621: DVU1621 - hypothetical protein (TIGR), at 1,705,135 to 1,705,557
DVU1621
DORF12360: DORF12360 - SEED: FIG00605053: hypothetical protein, at 1,705,703 to 1,706,176
DORF12360
DVU1622: purQ - phosphoribosylformylglycinamidine synthase I (TIGR), at 1,706,229 to 1,707,038
purQ
Position (kb)
1705
1706
1707 Strain fitness (log2 ratio)
-2
-1
0
1 at 1704.914 kb on + strand, within DVU1620 at 1704.937 kb on + strand, within DVU1620 at 1705.077 kb on + strand at 1705.193 kb on - strand, within DVU1621 at 1705.337 kb on + strand, within DVU1621 at 1705.566 kb on + strand at 1705.592 kb on + strand at 1705.713 kb on + strand at 1705.720 kb on + strand at 1705.720 kb on + strand at 1705.745 kb on - strand at 1705.762 kb on - strand, within DORF12360 at 1705.824 kb on + strand, within DORF12360 at 1705.910 kb on - strand, within DORF12360 at 1705.944 kb on + strand, within DORF12360 at 1706.080 kb on - strand, within DORF12360 at 1707.103 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with Casaminos as (N) remove 1,704,914 + DVU1620 0.51 -0.6 1,704,937 + DVU1620 0.60 +0.2 1,705,077 + +0.5 1,705,193 - DVU1621 0.14 -0.8 1,705,337 + DVU1621 0.48 -0.2 1,705,566 + -0.7 1,705,592 + -0.5 1,705,713 + -0.1 1,705,720 + +0.2 1,705,720 + -0.2 1,705,745 - -1.2 1,705,762 - DORF12360 0.12 +0.4 1,705,824 + DORF12360 0.26 -0.2 1,705,910 - DORF12360 0.44 -1.9 1,705,944 + DORF12360 0.51 -1.1 1,706,080 - DORF12360 0.80 -1.4 1,707,103 + -1.8
Or see this region's nucleotide sequence