Experiment: 7H9 with Clofazimine 0.45 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv3550 and Rv3551 overlap by 1 nucleotides Rv3551 and Rv3552 overlap by 4 nucleotides Rv3552 and Rv3553 are separated by 97 nucleotides
Rv3550: Rv3550 - Probable enoyl-CoA hydratase EchA20 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase), at 3,989,153 to 3,989,896
Rv3550
Rv3551: Rv3551 - Possible CoA-transferase (alpha subunit), at 3,989,896 to 3,990,774
Rv3551
Rv3552: Rv3552 - Possible CoA-transferase (beta subunit), at 3,990,771 to 3,991,523
Rv3552
Rv3553: Rv3553 - Possible oxidoreductase, at 3,991,621 to 3,992,688
Rv3553
Position (kb) 3990
3991
3992 Strain fitness (log2 ratio) -1
0
1 at 3989.811 kb on - strand, within Rv3550 at 3989.831 kb on - strand at 3989.894 kb on + strand at 3989.894 kb on + strand at 3989.894 kb on + strand at 3989.894 kb on + strand at 3989.895 kb on - strand at 3990.049 kb on + strand, within Rv3551 at 3990.713 kb on - strand at 3990.716 kb on + strand at 3991.290 kb on + strand, within Rv3552 at 3991.310 kb on - strand, within Rv3552 at 3991.480 kb on + strand at 3991.621 kb on - strand at 3992.183 kb on - strand, within Rv3553
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Clofazimine 0.45 ug/mL remove 3,989,811 - Rv3550 0.88 +0.3 3,989,831 - -1.5 3,989,894 + -0.5 3,989,894 + +0.1 3,989,894 + -0.3 3,989,894 + -0.0 3,989,895 - -0.6 3,990,049 + Rv3551 0.17 -0.9 3,990,713 - +0.7 3,990,716 + -0.4 3,991,290 + Rv3552 0.69 +0.4 3,991,310 - Rv3552 0.72 +0.2 3,991,480 + +0.8 3,991,621 - -0.2 3,992,183 - Rv3553 0.53 +0.1
Or see this region's nucleotide sequence