Experiment: 7H9 with Clofazimine 0.072 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv3562 and Rv3563 overlap by 4 nucleotides Rv3563 and Rv3564 overlap by 4 nucleotides Rv3564 and Rv3565 overlap by 4 nucleotides
Rv3562: Rv3562 - Probable acyl-CoA dehydrogenase FadE31, at 4,003,161 to 4,004,294
Rv3562
Rv3563: Rv3563 - Probable acyl-CoA dehydrogenase FadE32, at 4,004,291 to 4,005,250
Rv3563
Rv3564: Rv3564 - Probable acyl-CoA dehydrogenase FadE33, at 4,005,247 to 4,006,203
Rv3564
Rv3565: Rv3565 - Possible aspartate aminotransferase AspB (transaminase A) (ASPAT) (glutamic--oxaloacetic transaminase) (glutamic--aspartic transaminase), at 4,006,200 to 4,007,366
Rv3565
Position (kb) 4005
4006
4007 Strain fitness (log2 ratio) -1
0
1 at 4004.787 kb on - strand, within Rv3563 at 4004.846 kb on + strand, within Rv3563 at 4004.847 kb on - strand, within Rv3563 at 4005.380 kb on + strand, within Rv3564 at 4005.381 kb on - strand, within Rv3564 at 4005.619 kb on + strand, within Rv3564 at 4005.937 kb on + strand, within Rv3564 at 4007.160 kb on + strand, within Rv3565 at 4007.160 kb on + strand, within Rv3565
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Clofazimine 0.072 ug/mL remove 4,004,787 - Rv3563 0.52 -1.4 4,004,846 + Rv3563 0.58 +1.4 4,004,847 - Rv3563 0.58 +0.1 4,005,380 + Rv3564 0.14 -1.5 4,005,381 - Rv3564 0.14 -0.4 4,005,619 + Rv3564 0.39 -0.3 4,005,937 + Rv3564 0.72 -1.2 4,007,160 + Rv3565 0.82 -0.5 4,007,160 + Rv3565 0.82 +1.4
Or see this region's nucleotide sequence