Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3231c

Experiment: 7H9 with Clofazimine 0.072 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3230c and Rv3231c are separated by 109 nucleotidesRv3231c and Rv3232c overlap by 4 nucleotidesRv3232c and Rv3233c are separated by 23 nucleotides Rv3230c: Rv3230c - Hypothetical oxidoreductase, at 3,607,112 to 3,608,254 Rv3230c Rv3231c: Rv3231c - Conserved protein, at 3,608,364 to 3,608,873 Rv3231c Rv3232c: Rv3232c - Polyphosphate kinase Ppk2 (polyphosphoric acid kinase), at 3,608,870 to 3,609,757 Rv3232c Rv3233c: Rv3233c - Possible triacylglycerol synthase (diacylglycerol acyltransferase), at 3,609,781 to 3,610,371 Rv3233c Position (kb) 3608 3609Strain fitness (log2 ratio) -1 0 1 2 3 4at 3607.659 kb on - strand, within Rv3230cat 3607.714 kb on + strand, within Rv3230cat 3607.714 kb on + strand, within Rv3230cat 3607.971 kb on + strand, within Rv3230cat 3607.972 kb on - strand, within Rv3230cat 3607.972 kb on - strand, within Rv3230cat 3608.113 kb on - strand, within Rv3230cat 3608.113 kb on - strand, within Rv3230cat 3608.255 kb on - strandat 3608.255 kb on - strandat 3608.276 kb on - strandat 3608.320 kb on + strandat 3608.324 kb on + strandat 3608.531 kb on - strand, within Rv3231cat 3608.531 kb on - strand, within Rv3231cat 3608.602 kb on + strand, within Rv3231cat 3608.603 kb on - strand, within Rv3231cat 3608.603 kb on - strand, within Rv3231cat 3608.755 kb on + strand, within Rv3231cat 3608.756 kb on - strand, within Rv3231cat 3608.860 kb on + strandat 3608.861 kb on - strandat 3608.895 kb on + strandat 3608.895 kb on + strandat 3608.896 kb on - strandat 3608.896 kb on - strandat 3608.896 kb on - strandat 3608.896 kb on - strandat 3608.907 kb on + strandat 3608.908 kb on - strandat 3608.908 kb on - strandat 3608.937 kb on + strandat 3608.937 kb on + strandat 3608.997 kb on + strand, within Rv3232cat 3608.998 kb on - strand, within Rv3232cat 3609.069 kb on + strand, within Rv3232cat 3609.069 kb on + strand, within Rv3232cat 3609.070 kb on - strand, within Rv3232cat 3609.070 kb on - strand, within Rv3232cat 3609.127 kb on - strand, within Rv3232cat 3609.318 kb on + strand, within Rv3232cat 3609.318 kb on + strand, within Rv3232cat 3609.319 kb on - strand, within Rv3232cat 3609.319 kb on - strand, within Rv3232cat 3609.331 kb on + strand, within Rv3232cat 3609.370 kb on - strand, within Rv3232cat 3609.392 kb on + strand, within Rv3232cat 3609.392 kb on + strand, within Rv3232cat 3609.393 kb on - strand, within Rv3232cat 3609.393 kb on - strand, within Rv3232cat 3609.421 kb on + strand, within Rv3232cat 3609.421 kb on + strand, within Rv3232cat 3609.422 kb on - strand, within Rv3232cat 3609.423 kb on + strand, within Rv3232cat 3609.423 kb on + strand, within Rv3232cat 3609.423 kb on + strand, within Rv3232cat 3609.423 kb on + strand, within Rv3232cat 3609.424 kb on - strand, within Rv3232cat 3609.432 kb on + strand, within Rv3232cat 3609.432 kb on + strand, within Rv3232cat 3609.432 kb on + strand, within Rv3232cat 3609.433 kb on - strand, within Rv3232cat 3609.433 kb on - strand, within Rv3232cat 3609.435 kb on + strand, within Rv3232cat 3609.504 kb on + strand, within Rv3232cat 3609.536 kb on - strand, within Rv3232cat 3609.645 kb on + strand, within Rv3232cat 3609.749 kb on + strandat 3609.749 kb on + strandat 3609.750 kb on - strandat 3609.752 kb on - strandat 3609.783 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Clofazimine 0.072 ug/mL
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3,607,659 - Rv3230c 0.48 +3.9
3,607,714 + Rv3230c 0.53 +0.1
3,607,714 + Rv3230c 0.53 +1.9
3,607,971 + Rv3230c 0.75 +0.3
3,607,972 - Rv3230c 0.75 -0.6
3,607,972 - Rv3230c 0.75 -0.6
3,608,113 - Rv3230c 0.88 +0.2
3,608,113 - Rv3230c 0.88 +2.9
3,608,255 - -0.7
3,608,255 - -1.4
3,608,276 - +0.0
3,608,320 + +0.2
3,608,324 + -1.0
3,608,531 - Rv3231c 0.33 +0.2
3,608,531 - Rv3231c 0.33 +0.1
3,608,602 + Rv3231c 0.47 +0.2
3,608,603 - Rv3231c 0.47 +0.4
3,608,603 - Rv3231c 0.47 +0.1
3,608,755 + Rv3231c 0.77 +0.1
3,608,756 - Rv3231c 0.77 +0.5
3,608,860 + -0.2
3,608,861 - +1.9
3,608,895 + +0.3
3,608,895 + +0.7
3,608,896 - -0.3
3,608,896 - -0.2
3,608,896 - -1.2
3,608,896 - +0.0
3,608,907 + -0.5
3,608,908 - -0.2
3,608,908 - +1.4
3,608,937 + +0.1
3,608,937 + -0.3
3,608,997 + Rv3232c 0.14 -0.2
3,608,998 - Rv3232c 0.14 -0.3
3,609,069 + Rv3232c 0.22 -0.2
3,609,069 + Rv3232c 0.22 -0.1
3,609,070 - Rv3232c 0.23 +0.5
3,609,070 - Rv3232c 0.23 -0.7
3,609,127 - Rv3232c 0.29 -0.8
3,609,318 + Rv3232c 0.50 -0.1
3,609,318 + Rv3232c 0.50 -0.6
3,609,319 - Rv3232c 0.51 +0.2
3,609,319 - Rv3232c 0.51 -1.2
3,609,331 + Rv3232c 0.52 -0.2
3,609,370 - Rv3232c 0.56 +1.5
3,609,392 + Rv3232c 0.59 -0.8
3,609,392 + Rv3232c 0.59 +2.2
3,609,393 - Rv3232c 0.59 +0.1
3,609,393 - Rv3232c 0.59 +1.9
3,609,421 + Rv3232c 0.62 +0.4
3,609,421 + Rv3232c 0.62 +0.0
3,609,422 - Rv3232c 0.62 +0.1
3,609,423 + Rv3232c 0.62 -0.6
3,609,423 + Rv3232c 0.62 +0.7
3,609,423 + Rv3232c 0.62 +0.6
3,609,423 + Rv3232c 0.62 +0.2
3,609,424 - Rv3232c 0.62 -0.9
3,609,432 + Rv3232c 0.63 -0.6
3,609,432 + Rv3232c 0.63 +0.9
3,609,432 + Rv3232c 0.63 -0.0
3,609,433 - Rv3232c 0.63 +0.3
3,609,433 - Rv3232c 0.63 +0.1
3,609,435 + Rv3232c 0.64 +1.1
3,609,504 + Rv3232c 0.71 +0.2
3,609,536 - Rv3232c 0.75 -0.0
3,609,645 + Rv3232c 0.87 -0.3
3,609,749 + -0.4
3,609,749 + -0.2
3,609,750 - -0.3
3,609,752 - +0.4
3,609,783 - +0.4

Or see this region's nucleotide sequence