Experiment: Lactate-Sulfate (60-30 mM) + 0.1% Yeast Extract (MoYLS4)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU3241 and rpoZ are separated by 402 nucleotides rpoZ and dnaJ are separated by 12 nucleotides dnaJ and greA are separated by 376 nucleotides
DVU3241: DVU3241 - conserved hypothetical protein (TIGR), at 3,411,881 to 3,412,627
DVU3241
DVU3242: rpoZ - DNA-directed RNA polymerase, omega subunit (TIGR), at 3,413,030 to 3,413,263
rpoZ
DVU3243: dnaJ - dnaJ protein (TIGR), at 3,413,276 to 3,414,406
dnaJ
DVU3245: greA - transcription elongation factor GreA (TIGR), at 3,414,783 to 3,415,274
greA
Position (kb)
3413
3414
3415 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3412.281 kb on - strand, within DVU3241 at 3412.289 kb on - strand, within DVU3241 at 3412.289 kb on - strand, within DVU3241 at 3412.289 kb on - strand, within DVU3241 at 3412.315 kb on - strand, within DVU3241 at 3412.338 kb on - strand, within DVU3241 at 3412.348 kb on + strand, within DVU3241 at 3412.372 kb on + strand, within DVU3241 at 3412.380 kb on - strand, within DVU3241 at 3412.385 kb on - strand, within DVU3241 at 3412.385 kb on - strand, within DVU3241 at 3412.403 kb on - strand, within DVU3241 at 3412.403 kb on - strand, within DVU3241 at 3412.416 kb on - strand, within DVU3241 at 3412.468 kb on + strand, within DVU3241 at 3412.488 kb on - strand, within DVU3241 at 3412.492 kb on + strand, within DVU3241 at 3412.519 kb on + strand, within DVU3241 at 3412.527 kb on - strand, within DVU3241 at 3412.532 kb on - strand, within DVU3241 at 3412.712 kb on + strand at 3412.751 kb on + strand at 3412.759 kb on - strand at 3412.780 kb on - strand at 3412.793 kb on - strand at 3412.878 kb on + strand at 3412.884 kb on - strand at 3412.942 kb on - strand at 3412.972 kb on - strand at 3412.972 kb on - strand at 3413.300 kb on + strand at 3413.368 kb on + strand at 3413.431 kb on + strand, within dnaJ at 3413.772 kb on - strand, within dnaJ at 3413.803 kb on - strand, within dnaJ at 3413.831 kb on + strand, within dnaJ at 3413.831 kb on + strand, within dnaJ at 3413.836 kb on + strand, within dnaJ at 3413.839 kb on - strand, within dnaJ at 3413.925 kb on - strand, within dnaJ at 3413.953 kb on + strand, within dnaJ at 3414.031 kb on + strand, within dnaJ at 3414.049 kb on + strand, within dnaJ at 3414.057 kb on - strand, within dnaJ at 3414.228 kb on + strand, within dnaJ at 3414.228 kb on + strand, within dnaJ at 3414.290 kb on - strand, within dnaJ at 3414.314 kb on - strand at 3414.366 kb on - strand at 3414.388 kb on + strand at 3414.390 kb on - strand at 3414.424 kb on - strand at 3414.509 kb on - strand at 3414.566 kb on + strand at 3414.597 kb on + strand at 3414.677 kb on + strand at 3414.700 kb on + strand at 3414.700 kb on + strand at 3414.771 kb on - strand at 3414.992 kb on + strand, within greA at 3414.995 kb on - strand, within greA at 3415.000 kb on - strand, within greA at 3415.031 kb on + strand, within greA at 3415.096 kb on - strand, within greA at 3415.136 kb on - strand, within greA at 3415.171 kb on + strand, within greA at 3415.175 kb on + strand, within greA at 3415.202 kb on - strand, within greA at 3415.253 kb on + strand at 3415.253 kb on + strand at 3415.260 kb on + strand at 3415.260 kb on + strand at 3415.276 kb on - strand at 3415.367 kb on - strand at 3415.374 kb on - strand at 3415.400 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Lactate-Sulfate (60-30 mM) + 0.1% Yeast Extract (MoYLS4) remove 3,412,281 - DVU3241 0.54 +0.0 3,412,289 - DVU3241 0.55 -0.3 3,412,289 - DVU3241 0.55 -0.1 3,412,289 - DVU3241 0.55 -0.5 3,412,315 - DVU3241 0.58 -1.1 3,412,338 - DVU3241 0.61 -0.1 3,412,348 + DVU3241 0.63 -1.2 3,412,372 + DVU3241 0.66 -0.2 3,412,380 - DVU3241 0.67 -0.5 3,412,385 - DVU3241 0.67 -0.1 3,412,385 - DVU3241 0.67 +0.5 3,412,403 - DVU3241 0.70 +0.5 3,412,403 - DVU3241 0.70 -0.1 3,412,416 - DVU3241 0.72 -0.0 3,412,468 + DVU3241 0.79 +0.6 3,412,488 - DVU3241 0.81 -0.8 3,412,492 + DVU3241 0.82 +0.3 3,412,519 + DVU3241 0.85 -0.3 3,412,527 - DVU3241 0.86 -0.4 3,412,532 - DVU3241 0.87 -0.4 3,412,712 + -0.3 3,412,751 + -0.2 3,412,759 - -0.5 3,412,780 - +1.2 3,412,793 - -0.0 3,412,878 + -0.2 3,412,884 - -0.6 3,412,942 - -0.5 3,412,972 - -0.7 3,412,972 - -0.6 3,413,300 + -3.3 3,413,368 + +0.6 3,413,431 + dnaJ DVU3243 0.14 +0.1 3,413,772 - dnaJ DVU3243 0.44 -0.9 3,413,803 - dnaJ DVU3243 0.47 -0.6 3,413,831 + dnaJ DVU3243 0.49 -0.4 3,413,831 + dnaJ DVU3243 0.49 +1.7 3,413,836 + dnaJ DVU3243 0.50 -1.4 3,413,839 - dnaJ DVU3243 0.50 -0.7 3,413,925 - dnaJ DVU3243 0.57 -0.6 3,413,953 + dnaJ DVU3243 0.60 +0.7 3,414,031 + dnaJ DVU3243 0.67 +0.1 3,414,049 + dnaJ DVU3243 0.68 -0.1 3,414,057 - dnaJ DVU3243 0.69 +0.7 3,414,228 + dnaJ DVU3243 0.84 -1.1 3,414,228 + dnaJ DVU3243 0.84 -0.4 3,414,290 - dnaJ DVU3243 0.90 -1.2 3,414,314 - +0.5 3,414,366 - -0.5 3,414,388 + -0.1 3,414,390 - +0.5 3,414,424 - -0.1 3,414,509 - -0.1 3,414,566 + -1.1 3,414,597 + -1.5 3,414,677 + +0.4 3,414,700 + -1.1 3,414,700 + -0.8 3,414,771 - -0.2 3,414,992 + greA DVU3245 0.42 -1.1 3,414,995 - greA DVU3245 0.43 +0.9 3,415,000 - greA DVU3245 0.44 -0.9 3,415,031 + greA DVU3245 0.50 -0.7 3,415,096 - greA DVU3245 0.64 -0.7 3,415,136 - greA DVU3245 0.72 -0.4 3,415,171 + greA DVU3245 0.79 +0.2 3,415,175 + greA DVU3245 0.80 -0.1 3,415,202 - greA DVU3245 0.85 -0.8 3,415,253 + -0.2 3,415,253 + +0.6 3,415,260 + -0.8 3,415,260 + -0.4 3,415,276 - -1.1 3,415,367 - +0.3 3,415,374 - +0.4 3,415,400 - +0.1
Or see this region's nucleotide sequence