Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3647c

Experiment: 7H9 with Linezolid 0.44 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3646c and Rv3647c are separated by 352 nucleotidesRv3647c and Rv3648c are separated by 139 nucleotidesRv3648c and Rv3649 are separated by 249 nucleotides Rv3646c: Rv3646c - DNA topoisomerase I TopA (omega-protein) (relaxing enzyme) (untwisting enzyme) (swivelase) (type I DNA topoisomerase) (nicking-closing enzyme) (TOPO I), at 4,084,453 to 4,087,257 Rv3646c Rv3647c: Rv3647c - Conserved hypothetical protein, at 4,087,610 to 4,088,188 Rv3647c Rv3648c: Rv3648c - Probable cold shock protein A CspA, at 4,088,328 to 4,088,531 Rv3648c Rv3649: Rv3649 - Probable helicase, at 4,088,781 to 4,091,096 Rv3649 Position (kb) 4087 4088 4089Strain fitness (log2 ratio) -1 0 1 2at 4087.400 kb on - strandat 4087.497 kb on + strandat 4087.498 kb on - strandat 4087.498 kb on - strandat 4087.628 kb on + strandat 4087.628 kb on + strandat 4087.628 kb on + strandat 4087.629 kb on - strandat 4087.629 kb on - strandat 4088.070 kb on - strand, within Rv3647cat 4088.154 kb on - strandat 4088.154 kb on - strandat 4088.227 kb on - strandat 4088.270 kb on + strandat 4088.270 kb on + strandat 4088.271 kb on - strandat 4088.271 kb on - strandat 4088.766 kb on - strandat 4088.766 kb on - strandat 4088.991 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Linezolid 0.44 ug/mL
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4,087,400 - -0.4
4,087,497 + -0.4
4,087,498 - -0.6
4,087,498 - +0.3
4,087,628 + -0.7
4,087,628 + +0.8
4,087,628 + -0.2
4,087,629 - -1.2
4,087,629 - +0.1
4,088,070 - Rv3647c 0.79 -0.0
4,088,154 - -0.8
4,088,154 - -0.8
4,088,227 - +1.8
4,088,270 + +1.3
4,088,270 + -0.5
4,088,271 - +1.8
4,088,271 - -0.1
4,088,766 - -0.8
4,088,766 - -0.4
4,088,991 + -1.0

Or see this region's nucleotide sequence