Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3255c

Experiment: 7H9 with Linezolid 0.44 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3254 and Rv3255c overlap by 23 nucleotidesRv3255c and Rv3256c are separated by 7 nucleotides Rv3254: Rv3254 - Conserved hypothetical protein, at 3,633,675 to 3,635,063 Rv3254 Rv3255c: Rv3255c - Probable mannose-6-phosphate isomerase ManA (phosphomannose isomerase) (phosphomannoisomerase) (PMI) (phosphohexoisomerase) (phosphohexomutase), at 3,635,041 to 3,636,267 Rv3255c Rv3256c: Rv3256c - Conserved protein, at 3,636,275 to 3,637,315 Rv3256c Position (kb) 3635 3636 3637Strain fitness (log2 ratio) -2 -1 0 1 2at 3634.172 kb on - strand, within Rv3254at 3634.284 kb on + strand, within Rv3254at 3634.386 kb on + strand, within Rv3254at 3634.387 kb on - strand, within Rv3254at 3634.688 kb on - strand, within Rv3254at 3634.718 kb on + strand, within Rv3254at 3634.718 kb on + strand, within Rv3254at 3634.770 kb on + strand, within Rv3254at 3634.771 kb on - strand, within Rv3254at 3634.950 kb on + strandat 3634.951 kb on - strandat 3636.826 kb on - strand, within Rv3256cat 3637.083 kb on - strand, within Rv3256c

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Linezolid 0.44 ug/mL
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3,634,172 - Rv3254 0.36 -0.7
3,634,284 + Rv3254 0.44 +0.1
3,634,386 + Rv3254 0.51 +0.2
3,634,387 - Rv3254 0.51 -0.0
3,634,688 - Rv3254 0.73 +1.3
3,634,718 + Rv3254 0.75 -2.0
3,634,718 + Rv3254 0.75 -1.4
3,634,770 + Rv3254 0.79 +0.6
3,634,771 - Rv3254 0.79 +0.5
3,634,950 + +1.0
3,634,951 - -0.1
3,636,826 - Rv3256c 0.53 +2.4
3,637,083 - Rv3256c 0.78 -0.1

Or see this region's nucleotide sequence