Experiment: Lactate-Sulfate (60-50 mM) minimal (LS4D)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt bioF and bioD overlap by 19 nucleotides bioD and lysA-2 are separated by 155 nucleotides lysA-2 and lysX overlap by 1 nucleotides
DVU2564: bioF - 8-amino-7-oxononanoate synthase (Dmitry Rodionov), at 2,676,659 to 2,677,933
bioF
DVU2565: bioD - dethiobiotin synthetase (TIGR), at 2,677,915 to 2,678,655
bioD
DVU2566: lysA-2 - diaminopimelate decarboxylase (TIGR), at 2,678,811 to 2,680,076
lysA-2
DVU2567: lysX - predicted transcriptional regulator for lysine biosynthesis and transport (Dmitry Rodionov), at 2,680,076 to 2,680,780
lysX
Position (kb)
2678
2679
2680
2681 Strain fitness (log2 ratio)
-1
0
1 at 2677.957 kb on + strand at 2678.031 kb on + strand, within bioD at 2678.031 kb on + strand, within bioD at 2678.036 kb on + strand, within bioD at 2678.039 kb on - strand, within bioD at 2678.052 kb on + strand, within bioD at 2678.060 kb on - strand, within bioD at 2678.060 kb on - strand, within bioD at 2678.266 kb on - strand, within bioD at 2678.288 kb on - strand, within bioD at 2678.298 kb on - strand, within bioD at 2678.365 kb on + strand, within bioD at 2678.373 kb on + strand, within bioD at 2678.403 kb on - strand, within bioD at 2678.474 kb on - strand, within bioD at 2678.493 kb on + strand, within bioD at 2678.508 kb on - strand, within bioD at 2678.549 kb on + strand, within bioD at 2678.616 kb on - strand at 2678.659 kb on + strand at 2678.659 kb on + strand at 2678.660 kb on - strand at 2678.667 kb on - strand at 2678.667 kb on - strand at 2678.667 kb on - strand at 2678.667 kb on - strand at 2678.693 kb on - strand at 2678.724 kb on + strand at 2678.757 kb on + strand at 2678.794 kb on + strand at 2679.679 kb on + strand, within lysA-2 at 2679.687 kb on - strand, within lysA-2 at 2679.735 kb on + strand, within lysA-2 at 2679.743 kb on - strand, within lysA-2 at 2679.832 kb on + strand, within lysA-2 at 2679.862 kb on + strand, within lysA-2 at 2679.862 kb on + strand, within lysA-2 at 2679.992 kb on - strand at 2679.993 kb on + strand at 2680.074 kb on - strand at 2680.126 kb on + strand at 2680.143 kb on + strand at 2680.223 kb on + strand, within lysX at 2680.563 kb on - strand, within lysX at 2680.606 kb on - strand, within lysX at 2680.697 kb on + strand, within lysX at 2680.698 kb on + strand, within lysX at 2680.719 kb on + strand at 2680.794 kb on + strand at 2680.795 kb on - strand at 2680.838 kb on + strand at 2680.849 kb on + strand at 2680.870 kb on + strand at 2680.880 kb on + strand at 2680.880 kb on + strand at 2680.880 kb on + strand at 2680.880 kb on + strand at 2680.888 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Lactate-Sulfate (60-50 mM) minimal (LS4D) remove 2,677,957 + -0.5 2,678,031 + bioD DVU2565 0.16 -0.2 2,678,031 + bioD DVU2565 0.16 -1.0 2,678,036 + bioD DVU2565 0.16 +0.2 2,678,039 - bioD DVU2565 0.17 -0.4 2,678,052 + bioD DVU2565 0.18 +0.7 2,678,060 - bioD DVU2565 0.20 -0.6 2,678,060 - bioD DVU2565 0.20 -0.9 2,678,266 - bioD DVU2565 0.47 +0.5 2,678,288 - bioD DVU2565 0.50 +0.6 2,678,298 - bioD DVU2565 0.52 -0.9 2,678,365 + bioD DVU2565 0.61 +0.2 2,678,373 + bioD DVU2565 0.62 -1.2 2,678,403 - bioD DVU2565 0.66 -0.4 2,678,474 - bioD DVU2565 0.75 +0.7 2,678,493 + bioD DVU2565 0.78 +0.1 2,678,508 - bioD DVU2565 0.80 -0.2 2,678,549 + bioD DVU2565 0.86 -0.6 2,678,616 - -0.2 2,678,659 + +0.1 2,678,659 + +0.3 2,678,660 - -0.3 2,678,667 - +0.3 2,678,667 - -0.1 2,678,667 - -0.3 2,678,667 - -0.7 2,678,693 - +0.0 2,678,724 + +1.6 2,678,757 + +0.6 2,678,794 + -0.1 2,679,679 + lysA-2 DVU2566 0.69 -0.7 2,679,687 - lysA-2 DVU2566 0.69 +0.1 2,679,735 + lysA-2 DVU2566 0.73 -1.7 2,679,743 - lysA-2 DVU2566 0.74 +0.9 2,679,832 + lysA-2 DVU2566 0.81 -1.5 2,679,862 + lysA-2 DVU2566 0.83 -1.7 2,679,862 + lysA-2 DVU2566 0.83 -0.7 2,679,992 - -0.9 2,679,993 + -0.9 2,680,074 - -0.4 2,680,126 + -0.2 2,680,143 + +0.1 2,680,223 + lysX DVU2567 0.21 +0.4 2,680,563 - lysX DVU2567 0.69 -0.4 2,680,606 - lysX DVU2567 0.75 -0.0 2,680,697 + lysX DVU2567 0.88 -0.0 2,680,698 + lysX DVU2567 0.88 +0.5 2,680,719 + +0.3 2,680,794 + +1.1 2,680,795 - +1.1 2,680,838 + -0.2 2,680,849 + -0.4 2,680,870 + -0.5 2,680,880 + +0.6 2,680,880 + +0.1 2,680,880 + +0.2 2,680,880 + -0.2 2,680,888 - -0.7
Or see this region's nucleotide sequence