Experiment: 7H9 with Ethambutol 0.75 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0542c and Rv0543c are separated by 68 nucleotides Rv0543c and Rv0544c are separated by 59 nucleotides Rv0544c and Rv0545c overlap by 4 nucleotides
Rv0542c: Rv0542c - Possible O-succinylbenzoic acid--CoA ligase MenE (OSB-CoA synthetase) (O-succinylbenzoate-CoA synthase), at 634,416 to 635,504
Rv0542c
Rv0543c: Rv0543c - Conserved protein, at 635,573 to 635,875
Rv0543c
Rv0544c: Rv0544c - Possible conserved transmembrane protein, at 635,935 to 636,213
Rv0544c
Rv0545c: Rv0545c - Probable low-affinity inorganic phosphate transporter integral membrane protein PitA, at 636,210 to 637,463
Rv0545c
Position (kb) 635
636 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 635.882 kb on - strand at 635.882 kb on - strand at 635.882 kb on - strand at 635.884 kb on - strand at 635.936 kb on + strand at 635.999 kb on + strand, within Rv0544c at 635.999 kb on + strand, within Rv0544c at 636.020 kb on - strand, within Rv0544c at 636.195 kb on - strand at 636.195 kb on - strand at 636.195 kb on - strand at 636.763 kb on + strand, within Rv0545c at 636.764 kb on - strand, within Rv0545c
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Ethambutol 0.75 ug/mL remove 635,882 - -1.9 635,882 - -1.0 635,882 - +0.4 635,884 - -0.5 635,936 + +2.9 635,999 + Rv0544c 0.23 +1.2 635,999 + Rv0544c 0.23 +1.9 636,020 - Rv0544c 0.30 +0.7 636,195 - +1.0 636,195 - +0.9 636,195 - +1.6 636,763 + Rv0545c 0.44 +1.4 636,764 - Rv0545c 0.44 +1.2
Or see this region's nucleotide sequence