Experiment: 7H9 with Ethambutol 0.75 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0078 and Rv0078A are separated by 74 nucleotides Rv0078A and Rv0078B overlap by 4 nucleotides Rv0078B and Rv0079 are separated by 199 nucleotides
Rv0078: Rv0078 - Probable transcriptional regulatory protein, at 86,528 to 87,133
Rv0078
Rv0078A: Rv0078A - Hypothetical protein, at 87,208 to 87,801
Rv0078A
Rv0078B: Rv0078B - Conserved protein, at 87,798 to 88,004
Rv0078B
Rv0079: Rv0079 - Unknown protein, at 88,204 to 89,025
Rv0079
Position (kb) 87
88
89 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 86.836 kb on - strand, within Rv0078 at 86.925 kb on - strand, within Rv0078 at 87.125 kb on - strand at 87.276 kb on + strand, within Rv0078A at 87.289 kb on - strand, within Rv0078A at 87.343 kb on + strand, within Rv0078A at 87.605 kb on + strand, within Rv0078A at 87.606 kb on - strand, within Rv0078A at 87.632 kb on - strand, within Rv0078A at 87.955 kb on + strand, within Rv0078B at 87.955 kb on + strand, within Rv0078B at 87.955 kb on + strand, within Rv0078B at 87.955 kb on + strand, within Rv0078B at 87.955 kb on + strand, within Rv0078B at 88.233 kb on - strand at 88.233 kb on - strand at 88.280 kb on + strand at 88.440 kb on - strand, within Rv0079 at 88.631 kb on + strand, within Rv0079 at 88.681 kb on + strand, within Rv0079 at 88.682 kb on - strand, within Rv0079 at 88.732 kb on + strand, within Rv0079 at 88.779 kb on - strand, within Rv0079 at 88.779 kb on - strand, within Rv0079 at 88.954 kb on + strand at 88.954 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Ethambutol 0.75 ug/mL remove 86,836 - Rv0078 0.51 -1.8 86,925 - Rv0078 0.66 +1.1 87,125 - -0.3 87,276 + Rv0078A 0.11 -0.3 87,289 - Rv0078A 0.14 -0.3 87,343 + Rv0078A 0.23 -0.3 87,605 + Rv0078A 0.67 -0.5 87,606 - Rv0078A 0.67 -0.6 87,632 - Rv0078A 0.71 +0.1 87,955 + Rv0078B 0.76 -0.0 87,955 + Rv0078B 0.76 -2.5 87,955 + Rv0078B 0.76 -0.7 87,955 + Rv0078B 0.76 -0.4 87,955 + Rv0078B 0.76 -1.5 88,233 - +0.0 88,233 - +0.9 88,280 + -0.5 88,440 - Rv0079 0.29 +0.5 88,631 + Rv0079 0.52 -0.4 88,681 + Rv0079 0.58 +0.9 88,682 - Rv0079 0.58 +0.3 88,732 + Rv0079 0.64 +0.4 88,779 - Rv0079 0.70 +0.0 88,779 - Rv0079 0.70 -2.8 88,954 + -0.2 88,954 + -0.3
Or see this region's nucleotide sequence