Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3341

Experiment: 7H9 with Ethambutol 0.75 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3340 and Rv3341 are separated by 11 nucleotidesRv3341 and Rv3342 overlap by 4 nucleotidesRv3342 and Rv3343c are separated by 8 nucleotides Rv3340: Rv3340 - Probable O-acetylhomoserine sulfhydrylase MetC (homocysteine synthase) (O-acetylhomoserine (thiol)-lyase) (OAH sulfhydrylase) (O-acetyl-L-homoserine sulfhydrylase), at 3,726,127 to 3,727,476 Rv3340 Rv3341: Rv3341 - Probable homoserine O-acetyltransferase MetA (homoserine O-trans-acetylase) (homoserine transacetylase) (HTA), at 3,727,488 to 3,728,627 Rv3341 Rv3342: Rv3342 - Possible methyltransferase (methylase), at 3,728,624 to 3,729,355 Rv3342 Rv3343c: Rv3343c - PPE family protein PPE54, at 3,729,364 to 3,736,935 Rv3343c Position (kb) 3727 3728 3729Strain fitness (log2 ratio) -1 0 1at 3726.502 kb on + strand, within Rv3340at 3727.111 kb on + strand, within Rv3340at 3727.111 kb on + strand, within Rv3340at 3727.111 kb on + strand, within Rv3340at 3727.389 kb on + strandat 3727.504 kb on + strandat 3727.504 kb on + strandat 3728.699 kb on + strand, within Rv3342at 3729.134 kb on - strand, within Rv3342at 3729.212 kb on + strand, within Rv3342at 3729.468 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Ethambutol 0.75 ug/mL
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3,726,502 + Rv3340 0.28 +0.0
3,727,111 + Rv3340 0.73 +0.2
3,727,111 + Rv3340 0.73 -0.1
3,727,111 + Rv3340 0.73 -0.3
3,727,389 + -0.7
3,727,504 + +0.1
3,727,504 + -0.2
3,728,699 + Rv3342 0.10 -0.5
3,729,134 - Rv3342 0.70 +0.9
3,729,212 + Rv3342 0.80 -0.5
3,729,468 - +0.7

Or see this region's nucleotide sequence