Experiment: 7H9 with Bedaquiline 0.15 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0663 and Rv0664 are separated by 31 nucleotides Rv0664 and Rv0665 overlap by 4 nucleotides Rv0665 and Rv0666 overlap by 4 nucleotides Rv0666 and Rv0667 are separated by 497 nucleotides
Rv0663: Rv0663 - Possible arylsulfatase AtsD (aryl-sulfate sulphohydrolase) (arylsulphatase), at 756,137 to 758,500
Rv0663
Rv0664: Rv0664 - Possible antitoxin VapB8, at 758,532 to 758,804
Rv0664
Rv0665: Rv0665 - Possible toxin VapC8, at 758,801 to 759,139
Rv0665
Rv0666: Rv0666 - Possible membrane protein, at 759,136 to 759,309
Rv0666
Rv0667: Rv0667 - DNA-directed RNA polymerase (beta chain) RpoB (transcriptase beta chain) (RNA polymerase beta subunit), at 759,807 to 763,325
Rv0667
Position (kb) 758
759
760 Strain fitness (log2 ratio) -2
-1
0
1 at 758.117 kb on + strand, within Rv0663 at 758.123 kb on + strand, within Rv0663 at 758.151 kb on - strand, within Rv0663 at 758.787 kb on - strand at 758.832 kb on + strand at 758.837 kb on - strand, within Rv0665 at 758.908 kb on + strand, within Rv0665 at 759.410 kb on + strand at 759.545 kb on + strand at 759.555 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Bedaquiline 0.15 ug/mL remove 758,117 + Rv0663 0.84 +0.2 758,123 + Rv0663 0.84 -1.7 758,151 - Rv0663 0.85 -0.9 758,787 - -0.5 758,832 + +0.9 758,837 - Rv0665 0.11 +0.5 758,908 + Rv0665 0.32 -0.0 759,410 + -0.9 759,545 + -2.0 759,555 + +0.2
Or see this region's nucleotide sequence