Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2224c

Experiment: 7H9 with Rifampicin 0.014 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2223c and Rv2224c are separated by 61 nucleotidesRv2224c and Rv2225 are separated by 718 nucleotides Rv2223c: Rv2223c - Probable exported protease, at 2,493,837 to 2,495,399 Rv2223c Rv2224c: Rv2224c - Probable carboxylesterase CaeA, at 2,495,461 to 2,497,023 Rv2224c Rv2225: Rv2225 - 3-methyl-2-oxobutanoate hydroxymethyltransferase PanB, at 2,497,742 to 2,498,587 Rv2225 Position (kb) 2495 2496 2497 2498Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 2494.487 kb on - strand, within Rv2223cat 2494.555 kb on + strand, within Rv2223cat 2494.555 kb on + strand, within Rv2223cat 2494.556 kb on - strand, within Rv2223cat 2494.629 kb on - strand, within Rv2223cat 2494.662 kb on - strand, within Rv2223cat 2494.703 kb on - strand, within Rv2223cat 2494.703 kb on - strand, within Rv2223cat 2494.703 kb on - strand, within Rv2223cat 2494.717 kb on + strand, within Rv2223cat 2494.717 kb on + strand, within Rv2223cat 2494.717 kb on + strand, within Rv2223cat 2495.028 kb on - strand, within Rv2223cat 2495.155 kb on + strand, within Rv2223cat 2495.156 kb on - strand, within Rv2223cat 2495.322 kb on + strandat 2495.415 kb on + strandat 2495.415 kb on + strandat 2495.416 kb on - strandat 2495.504 kb on + strandat 2495.545 kb on + strandat 2495.545 kb on + strandat 2495.546 kb on - strandat 2495.546 kb on - strandat 2495.621 kb on + strand, within Rv2224cat 2495.622 kb on - strand, within Rv2224cat 2495.622 kb on - strand, within Rv2224cat 2495.622 kb on - strand, within Rv2224cat 2495.762 kb on + strand, within Rv2224cat 2495.763 kb on - strand, within Rv2224cat 2495.909 kb on + strand, within Rv2224cat 2495.909 kb on + strand, within Rv2224cat 2495.909 kb on + strand, within Rv2224cat 2495.909 kb on + strand, within Rv2224cat 2495.909 kb on + strand, within Rv2224cat 2495.909 kb on + strand, within Rv2224cat 2495.910 kb on - strand, within Rv2224cat 2495.910 kb on - strand, within Rv2224cat 2495.910 kb on - strand, within Rv2224cat 2495.910 kb on - strand, within Rv2224cat 2495.999 kb on + strand, within Rv2224cat 2496.000 kb on - strand, within Rv2224cat 2496.010 kb on - strand, within Rv2224cat 2496.033 kb on + strand, within Rv2224cat 2496.033 kb on + strand, within Rv2224cat 2496.034 kb on - strand, within Rv2224cat 2496.035 kb on + strand, within Rv2224cat 2496.036 kb on - strand, within Rv2224cat 2496.123 kb on - strand, within Rv2224cat 2496.191 kb on + strand, within Rv2224cat 2496.192 kb on - strand, within Rv2224cat 2496.192 kb on - strand, within Rv2224cat 2496.192 kb on - strand, within Rv2224cat 2496.192 kb on - strand, within Rv2224cat 2496.315 kb on - strand, within Rv2224cat 2496.315 kb on - strand, within Rv2224cat 2496.339 kb on - strand, within Rv2224cat 2496.345 kb on - strand, within Rv2224cat 2496.345 kb on - strand, within Rv2224cat 2496.353 kb on + strand, within Rv2224cat 2496.516 kb on - strand, within Rv2224cat 2496.706 kb on + strand, within Rv2224cat 2496.818 kb on - strand, within Rv2224cat 2496.838 kb on + strand, within Rv2224cat 2496.838 kb on + strand, within Rv2224cat 2496.838 kb on + strand, within Rv2224cat 2496.839 kb on - strand, within Rv2224cat 2496.839 kb on - strand, within Rv2224cat 2496.903 kb on + strandat 2497.136 kb on + strandat 2497.136 kb on + strandat 2497.137 kb on - strandat 2497.137 kb on - strandat 2497.137 kb on - strandat 2497.623 kb on + strandat 2497.624 kb on - strandat 2497.624 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Rifampicin 0.014 ug/mL
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2,494,487 - Rv2223c 0.42 +0.3
2,494,555 + Rv2223c 0.46 -0.6
2,494,555 + Rv2223c 0.46 +2.7
2,494,556 - Rv2223c 0.46 +0.2
2,494,629 - Rv2223c 0.51 +1.6
2,494,662 - Rv2223c 0.53 +0.1
2,494,703 - Rv2223c 0.55 +0.9
2,494,703 - Rv2223c 0.55 -0.4
2,494,703 - Rv2223c 0.55 +0.3
2,494,717 + Rv2223c 0.56 -0.5
2,494,717 + Rv2223c 0.56 -0.2
2,494,717 + Rv2223c 0.56 +0.7
2,495,028 - Rv2223c 0.76 +1.7
2,495,155 + Rv2223c 0.84 -1.3
2,495,156 - Rv2223c 0.84 -0.5
2,495,322 + -0.2
2,495,415 + +0.2
2,495,415 + +0.3
2,495,416 - -1.9
2,495,504 + -2.0
2,495,545 + -1.8
2,495,545 + -0.9
2,495,546 - -3.4
2,495,546 - -2.9
2,495,621 + Rv2224c 0.10 -2.5
2,495,622 - Rv2224c 0.10 +0.1
2,495,622 - Rv2224c 0.10 -3.9
2,495,622 - Rv2224c 0.10 -2.0
2,495,762 + Rv2224c 0.19 -1.8
2,495,763 - Rv2224c 0.19 -3.3
2,495,909 + Rv2224c 0.29 -1.8
2,495,909 + Rv2224c 0.29 -2.5
2,495,909 + Rv2224c 0.29 -3.4
2,495,909 + Rv2224c 0.29 +0.7
2,495,909 + Rv2224c 0.29 -4.2
2,495,909 + Rv2224c 0.29 -3.6
2,495,910 - Rv2224c 0.29 -2.8
2,495,910 - Rv2224c 0.29 -3.1
2,495,910 - Rv2224c 0.29 -2.5
2,495,910 - Rv2224c 0.29 -2.8
2,495,999 + Rv2224c 0.34 -1.4
2,496,000 - Rv2224c 0.34 +1.1
2,496,010 - Rv2224c 0.35 -3.1
2,496,033 + Rv2224c 0.37 -3.1
2,496,033 + Rv2224c 0.37 -1.7
2,496,034 - Rv2224c 0.37 -0.6
2,496,035 + Rv2224c 0.37 -1.7
2,496,036 - Rv2224c 0.37 -1.9
2,496,123 - Rv2224c 0.42 -2.4
2,496,191 + Rv2224c 0.47 -2.4
2,496,192 - Rv2224c 0.47 -2.4
2,496,192 - Rv2224c 0.47 -1.8
2,496,192 - Rv2224c 0.47 +0.2
2,496,192 - Rv2224c 0.47 -3.3
2,496,315 - Rv2224c 0.55 -2.3
2,496,315 - Rv2224c 0.55 +1.7
2,496,339 - Rv2224c 0.56 -3.0
2,496,345 - Rv2224c 0.57 -2.0
2,496,345 - Rv2224c 0.57 -2.7
2,496,353 + Rv2224c 0.57 -1.4
2,496,516 - Rv2224c 0.67 -1.5
2,496,706 + Rv2224c 0.80 -1.1
2,496,818 - Rv2224c 0.87 -3.3
2,496,838 + Rv2224c 0.88 -2.9
2,496,838 + Rv2224c 0.88 -3.1
2,496,838 + Rv2224c 0.88 -2.2
2,496,839 - Rv2224c 0.88 -0.9
2,496,839 - Rv2224c 0.88 -3.5
2,496,903 + -2.2
2,497,136 + +0.0
2,497,136 + +1.1
2,497,137 - -0.6
2,497,137 - +0.4
2,497,137 - -0.2
2,497,623 + -0.7
2,497,624 - +1.7
2,497,624 - -0.1

Or see this region's nucleotide sequence