Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv1696

Experiment: 7H9 with Rifampicin 0.014 ug/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv1694 and Rv1695 overlap by 1 nucleotidesRv1695 and Rv1696 are separated by 13 nucleotidesRv1696 and Rv1697 are separated by 95 nucleotides Rv1694: Rv1694 - 2'-O-methyltransferase TlyA, at 1,917,940 to 1,918,746 Rv1694 Rv1695: Rv1695 - Inorganic polyphosphate/ATP-NAD kinase PpnK (poly(P)/ATP NAD kinase), at 1,918,746 to 1,919,669 Rv1695 Rv1696: Rv1696 - Probable DNA repair protein RecN (recombination protein N), at 1,919,683 to 1,921,446 Rv1696 Rv1697: Rv1697 - Conserved hypothetical protein, at 1,921,542 to 1,922,723 Rv1697 Position (kb) 1919 1920 1921 1922Strain fitness (log2 ratio) -3 -2 -1 0 1at 1919.813 kb on + strandat 1919.813 kb on + strandat 1919.887 kb on - strand, within Rv1696at 1920.038 kb on + strand, within Rv1696at 1920.179 kb on - strand, within Rv1696at 1920.332 kb on - strand, within Rv1696at 1920.388 kb on - strand, within Rv1696at 1920.571 kb on + strand, within Rv1696at 1920.655 kb on + strand, within Rv1696at 1920.655 kb on + strand, within Rv1696at 1921.413 kb on - strandat 1921.444 kb on + strandat 1921.445 kb on - strandat 1921.473 kb on - strandat 1921.475 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 7H9 with Rifampicin 0.014 ug/mL
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1,919,813 + +1.7
1,919,813 + -1.8
1,919,887 - Rv1696 0.12 -0.1
1,920,038 + Rv1696 0.20 -1.0
1,920,179 - Rv1696 0.28 -0.4
1,920,332 - Rv1696 0.37 -0.6
1,920,388 - Rv1696 0.40 -0.5
1,920,571 + Rv1696 0.50 -0.8
1,920,655 + Rv1696 0.55 -1.0
1,920,655 + Rv1696 0.55 +0.0
1,921,413 - -1.5
1,921,444 + -0.0
1,921,445 - -0.4
1,921,473 - -2.8
1,921,475 - -0.6

Or see this region's nucleotide sequence