Experiment: 7H9 with Rifampicin 0.014 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv0648 and Rv0649 overlap by 4 nucleotides Rv0649 and Rv0650 overlap by 1 nucleotides Rv0650 and Rv0651 are separated by 330 nucleotides Rv0651 and Rv0652 are separated by 36 nucleotides
Rv0648: Rv0648 - Alpha-mannosidase, at 742,719 to 746,366
Rv0648
Rv0649: Rv0649 - Possible malonyl CoA-acyl carrier protein transacylase FabD2 (MCT), at 746,363 to 747,037
Rv0649
Rv0650: Rv0650 - Possible sugar kinase, at 747,037 to 747,945
Rv0650
Rv0651: Rv0651 - 50S ribosomal protein L10 RplJ, at 748,276 to 748,812
Rv0651
Rv0652: Rv0652 - 50S ribosomal protein L7/L12 RplL (SA1), at 748,849 to 749,241
Rv0652
Position (kb) 747
748 Strain fitness (log2 ratio) -1
0
1 at 746.058 kb on + strand at 746.058 kb on + strand at 746.058 kb on + strand at 746.059 kb on - strand at 746.225 kb on - strand at 746.334 kb on + strand at 746.789 kb on + strand, within Rv0649 at 746.789 kb on + strand, within Rv0649 at 746.790 kb on - strand, within Rv0649 at 746.790 kb on - strand, within Rv0649 at 746.790 kb on - strand, within Rv0649 at 746.988 kb on - strand at 747.246 kb on + strand, within Rv0650 at 747.254 kb on + strand, within Rv0650 at 747.824 kb on + strand, within Rv0650 at 747.824 kb on + strand, within Rv0650 at 747.943 kb on + strand at 748.010 kb on - strand at 748.047 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Rifampicin 0.014 ug/mL remove 746,058 + -0.6 746,058 + -0.6 746,058 + -0.1 746,059 - +0.7 746,225 - -0.4 746,334 + +0.7 746,789 + Rv0649 0.63 -0.9 746,789 + Rv0649 0.63 -0.2 746,790 - Rv0649 0.63 -0.9 746,790 - Rv0649 0.63 -0.5 746,790 - Rv0649 0.63 -0.6 746,988 - -0.6 747,246 + Rv0650 0.23 -0.2 747,254 + Rv0650 0.24 -1.2 747,824 + Rv0650 0.87 -0.4 747,824 + Rv0650 0.87 -0.4 747,943 + -0.1 748,010 - +0.2 748,047 + -1.1
Or see this region's nucleotide sequence