Experiment: 7H9 with Rifampicin 0.014 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv3225c and Rv3226c are separated by 123 nucleotides Rv3226c and Rv3227 are separated by 54 nucleotides Rv3227 and Rv3228 overlap by 4 nucleotides
Rv3225c: Rv3225c - GCN5-related N-acetyltransferase, phosphorylase, at 3,601,016 to 3,602,440
Rv3225c
Rv3226c: Rv3226c - Conserved hypothetical protein, at 3,602,564 to 3,603,322
Rv3226c
Rv3227: Rv3227 - 3-phosphoshikimate 1-carboxyvinyltransferase AroA (5-enolpyruvylshikimate-3-phosphate synthase) (EPSP synthase) (EPSPS), at 3,603,377 to 3,604,729
Rv3227
Rv3228: Rv3228 - Conserved hypothetical protein, at 3,604,726 to 3,605,718
Rv3228
Position (kb) 3603
3604
3605 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 3602.847 kb on + strand, within Rv3226c at 3602.847 kb on + strand, within Rv3226c at 3602.848 kb on - strand, within Rv3226c at 3602.848 kb on - strand, within Rv3226c at 3603.043 kb on + strand, within Rv3226c at 3603.223 kb on - strand, within Rv3226c at 3603.223 kb on - strand, within Rv3226c at 3603.330 kb on - strand at 3603.330 kb on - strand at 3603.330 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 7H9 with Rifampicin 0.014 ug/mL remove 3,602,847 + Rv3226c 0.37 +0.8 3,602,847 + Rv3226c 0.37 -0.2 3,602,848 - Rv3226c 0.37 -3.8 3,602,848 - Rv3226c 0.37 +0.1 3,603,043 + Rv3226c 0.63 -0.3 3,603,223 - Rv3226c 0.87 +1.2 3,603,223 - Rv3226c 0.87 +2.8 3,603,330 - +0.3 3,603,330 - -0.1 3,603,330 - -0.2
Or see this region's nucleotide sequence