Experiment: no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rv3856c and Rv3857c are separated by 8 nucleotides Rv3857c and Rv3858c are separated by 424 nucleotides Rv3858c and Rv3859c overlap by 8 nucleotides
Rv3856c: Rv3856c - Conserved hypothetical protein, at 4,328,401 to 4,329,408
Rv3856c
Rv3857c: Rv3857c - Possible membrane protein, at 4,329,417 to 4,329,614
Rv3857c
Rv3858c: Rv3858c - Probable NADH-dependent glutamate synthase (small subunit) GltD (L-glutamate synthase) (L-glutamate synthetase) (NADH-glutamate synthase) (glutamate synthase (NADH)) (GLTS beta chain) (NADPH-GOGAT), at 4,330,039 to 4,331,505
Rv3858c
Rv3859c: Rv3859c - Probable ferredoxin-dependent glutamate synthase [NADPH] (large subunit) GltB (L-glutamate synthase) (L-glutamate synthetase) (NADH-glutamate synthase) (glutamate synthase (NADH))(NADPH-GOGAT), at 4,331,498 to 4,336,081
Rv3859c
Position (kb)
4330
4331
4332 Strain fitness (log2 ratio)
-1
0
1 at 4329.330 kb on - strand at 4329.371 kb on + strand at 4329.375 kb on - strand at 4329.495 kb on + strand, within Rv3857c at 4329.495 kb on + strand, within Rv3857c at 4329.565 kb on + strand, within Rv3857c at 4329.565 kb on + strand, within Rv3857c at 4329.566 kb on - strand, within Rv3857c at 4329.630 kb on - strand at 4329.630 kb on - strand at 4329.640 kb on + strand at 4329.641 kb on - strand at 4329.641 kb on - strand at 4329.735 kb on - strand at 4329.879 kb on + strand at 4329.929 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction no stress control remove 4,329,330 - -0.4 4,329,371 + -0.1 4,329,375 - +0.2 4,329,495 + Rv3857c 0.39 +0.3 4,329,495 + Rv3857c 0.39 -0.6 4,329,565 + Rv3857c 0.75 -0.2 4,329,565 + Rv3857c 0.75 -0.2 4,329,566 - Rv3857c 0.75 -0.4 4,329,630 - +0.8 4,329,630 - -0.1 4,329,640 + -1.1 4,329,641 - -0.2 4,329,641 - -0.3 4,329,735 - +1.5 4,329,879 + -0.3 4,329,929 + +0.0
Or see this region's nucleotide sequence