Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3858c

Experiment: no stress control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3856c and Rv3857c are separated by 8 nucleotidesRv3857c and Rv3858c are separated by 424 nucleotidesRv3858c and Rv3859c overlap by 8 nucleotides Rv3856c: Rv3856c - Conserved hypothetical protein, at 4,328,401 to 4,329,408 Rv3856c Rv3857c: Rv3857c - Possible membrane protein, at 4,329,417 to 4,329,614 Rv3857c Rv3858c: Rv3858c - Probable NADH-dependent glutamate synthase (small subunit) GltD (L-glutamate synthase) (L-glutamate synthetase) (NADH-glutamate synthase) (glutamate synthase (NADH)) (GLTS beta chain) (NADPH-GOGAT), at 4,330,039 to 4,331,505 Rv3858c Rv3859c: Rv3859c - Probable ferredoxin-dependent glutamate synthase [NADPH] (large subunit) GltB (L-glutamate synthase) (L-glutamate synthetase) (NADH-glutamate synthase) (glutamate synthase (NADH))(NADPH-GOGAT), at 4,331,498 to 4,336,081 Rv3859c Position (kb) 4330 4331 4332Strain fitness (log2 ratio) -1 0 1at 4329.330 kb on - strandat 4329.371 kb on + strandat 4329.375 kb on - strandat 4329.495 kb on + strand, within Rv3857cat 4329.495 kb on + strand, within Rv3857cat 4329.565 kb on + strand, within Rv3857cat 4329.565 kb on + strand, within Rv3857cat 4329.566 kb on - strand, within Rv3857cat 4329.630 kb on - strandat 4329.630 kb on - strandat 4329.640 kb on + strandat 4329.641 kb on - strandat 4329.641 kb on - strandat 4329.735 kb on - strandat 4329.879 kb on + strandat 4329.929 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction no stress control
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4,329,330 - -0.4
4,329,371 + -0.1
4,329,375 - +0.2
4,329,495 + Rv3857c 0.39 +0.3
4,329,495 + Rv3857c 0.39 -0.6
4,329,565 + Rv3857c 0.75 -0.2
4,329,565 + Rv3857c 0.75 -0.2
4,329,566 - Rv3857c 0.75 -0.4
4,329,630 - +0.8
4,329,630 - -0.1
4,329,640 + -1.1
4,329,641 - -0.2
4,329,641 - -0.3
4,329,735 - +1.5
4,329,879 + -0.3
4,329,929 + +0.0

Or see this region's nucleotide sequence