Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv3227

Experiment: no stress control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv3225c and Rv3226c are separated by 123 nucleotidesRv3226c and Rv3227 are separated by 54 nucleotidesRv3227 and Rv3228 overlap by 4 nucleotides Rv3225c: Rv3225c - GCN5-related N-acetyltransferase, phosphorylase, at 3,601,016 to 3,602,440 Rv3225c Rv3226c: Rv3226c - Conserved hypothetical protein, at 3,602,564 to 3,603,322 Rv3226c Rv3227: Rv3227 - 3-phosphoshikimate 1-carboxyvinyltransferase AroA (5-enolpyruvylshikimate-3-phosphate synthase) (EPSP synthase) (EPSPS), at 3,603,377 to 3,604,729 Rv3227 Rv3228: Rv3228 - Conserved hypothetical protein, at 3,604,726 to 3,605,718 Rv3228 Position (kb) 3603 3604 3605Strain fitness (log2 ratio) -1 0 1 2 3at 3602.847 kb on + strand, within Rv3226cat 3602.847 kb on + strand, within Rv3226cat 3602.848 kb on - strand, within Rv3226cat 3602.848 kb on - strand, within Rv3226cat 3603.043 kb on + strand, within Rv3226cat 3603.223 kb on - strand, within Rv3226cat 3603.223 kb on - strand, within Rv3226cat 3603.330 kb on - strandat 3603.330 kb on - strandat 3603.330 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction no stress control
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3,602,847 + Rv3226c 0.37 +0.9
3,602,847 + Rv3226c 0.37 -0.1
3,602,848 - Rv3226c 0.37 -1.4
3,602,848 - Rv3226c 0.37 -0.1
3,603,043 + Rv3226c 0.63 -0.2
3,603,223 - Rv3226c 0.87 +0.7
3,603,223 - Rv3226c 0.87 +2.9
3,603,330 - -1.0
3,603,330 - +0.4
3,603,330 - -0.0

Or see this region's nucleotide sequence