Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv2221c

Experiment: no stress control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv2220 and Rv2221c are separated by 317 nucleotidesRv2221c and Rv2222c are separated by 48 nucleotides Rv2220: Rv2220 - Glutamine synthetase GlnA1 (glutamine synthase) (GS-I), at 2,487,615 to 2,489,051 Rv2220 Rv2221c: Rv2221c - Glutamate-ammonia-ligase adenylyltransferase GlnE (glutamine-synthetase adenylyltransferase), at 2,489,369 to 2,492,353 Rv2221c Rv2222c: Rv2222c - Probable glutamine synthetase GlnA2 (glutamine synthase) (GS-II), at 2,492,402 to 2,493,742 Rv2222c Position (kb) 2489 2490 2491 2492 2493Strain fitness (log2 ratio) -2 -1 0 1at 2489.050 kb on - strandat 2489.050 kb on - strandat 2489.073 kb on + strandat 2489.174 kb on + strandat 2489.174 kb on + strandat 2489.315 kb on + strandat 2489.315 kb on + strandat 2489.316 kb on - strandat 2489.316 kb on - strandat 2490.904 kb on - strand, within Rv2221cat 2490.904 kb on - strand, within Rv2221cat 2491.242 kb on - strand, within Rv2221cat 2491.881 kb on - strand, within Rv2221cat 2492.404 kb on - strandat 2492.416 kb on - strandat 2492.416 kb on - strandat 2492.451 kb on + strandat 2492.619 kb on - strand, within Rv2222cat 2492.704 kb on - strand, within Rv2222cat 2492.732 kb on + strand, within Rv2222cat 2492.733 kb on - strand, within Rv2222cat 2492.770 kb on - strand, within Rv2222cat 2492.856 kb on + strand, within Rv2222cat 2493.252 kb on - strand, within Rv2222c

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Per-strain Table

Position Strand Gene LocusTag Fraction no stress control
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2,489,050 - -0.7
2,489,050 - -0.0
2,489,073 + -0.5
2,489,174 + -0.4
2,489,174 + -0.4
2,489,315 + -0.1
2,489,315 + -0.7
2,489,316 - -2.0
2,489,316 - -0.6
2,490,904 - Rv2221c 0.51 +1.1
2,490,904 - Rv2221c 0.51 +1.5
2,491,242 - Rv2221c 0.63 +0.2
2,491,881 - Rv2221c 0.84 -1.0
2,492,404 - +0.2
2,492,416 - +1.4
2,492,416 - -1.1
2,492,451 + +0.0
2,492,619 - Rv2222c 0.16 -0.2
2,492,704 - Rv2222c 0.23 +0.2
2,492,732 + Rv2222c 0.25 -1.8
2,492,733 - Rv2222c 0.25 +0.4
2,492,770 - Rv2222c 0.27 -1.0
2,492,856 + Rv2222c 0.34 -0.6
2,493,252 - Rv2222c 0.63 -0.5

Or see this region's nucleotide sequence