Strain Fitness in Mycobacterium tuberculosis H37Rv around Rv0005

Experiment: no stress control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRv0003 and Rv0004 overlap by 4 nucleotidesRv0004 and Rv0005 are separated by 242 nucleotidesRv0005 and Rv0006 are separated by 34 nucleotides Rv0003: Rv0003 - DNA replication and repair protein RecF (single-strand DNA binding protein), at 3,280 to 4,437 Rv0003 Rv0004: Rv0004 - Conserved hypothetical protein, at 4,434 to 4,997 Rv0004 Rv0005: Rv0005 - DNA gyrase (subunit B) GyrB (DNA topoisomerase (ATP-hydrolysing)) (DNA topoisomerase II) (type II DNA topoisomerase), at 5,240 to 7,267 Rv0005 Rv0006: Rv0006 - DNA gyrase (subunit A) GyrA (DNA topoisomerase (ATP-hydrolysing)) (DNA topoisomerase II) (type II DNA topoisomerase), at 7,302 to 9,818 Rv0006 Position (kb) 5 6 7 8Strain fitness (log2 ratio) -1 0 1at 5.066 kb on + strandat 5.128 kb on + strandat 7.299 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction no stress control
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5,066 + -0.4
5,128 + -0.2
7,299 + +1.0

Or see this region's nucleotide sequence