Experiment: Lactate-Sulfate (60-30 mM) minimal (MoLS4)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU2978 and DVU2979 are separated by 109 nucleotides DVU2979 and pssA are separated by 62 nucleotides pssA and leuA are separated by 176 nucleotides
DVU2978: DVU2978 - hydrolase, haloacid dehalogenase-like family (TIGR), at 3,085,457 to 3,086,089
DVU2978
DVU2979: DVU2979 - phosphatidylserine decarboxylase-related protein (TIGR), at 3,086,199 to 3,086,852
DVU2979
DVU2980: pssA - CDP-diacylglycerol--serine O-phosphatidyltransferase (TIGR), at 3,086,915 to 3,087,664
pssA
DVU2981: leuA - 2-isopropylmalate synthase (TIGR), at 3,087,841 to 3,089,370
leuA
Position (kb)
3086
3087
3088 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 3085.918 kb on - strand, within DVU2978 at 3085.952 kb on - strand, within DVU2978 at 3085.952 kb on - strand, within DVU2978 at 3086.085 kb on - strand at 3086.156 kb on - strand at 3086.816 kb on + strand at 3086.842 kb on - strand at 3086.854 kb on - strand at 3086.854 kb on - strand at 3086.854 kb on - strand at 3086.854 kb on - strand at 3087.654 kb on + strand at 3087.654 kb on + strand at 3087.724 kb on + strand at 3087.823 kb on + strand at 3087.866 kb on + strand at 3087.866 kb on + strand at 3087.866 kb on + strand at 3087.874 kb on - strand at 3087.884 kb on - strand at 3087.912 kb on + strand at 3087.942 kb on + strand at 3088.020 kb on - strand, within leuA at 3088.067 kb on - strand, within leuA at 3088.088 kb on + strand, within leuA at 3088.125 kb on + strand, within leuA at 3088.159 kb on + strand, within leuA at 3088.203 kb on + strand, within leuA at 3088.238 kb on + strand, within leuA at 3088.279 kb on + strand, within leuA at 3088.324 kb on + strand, within leuA at 3088.482 kb on + strand, within leuA at 3088.572 kb on + strand, within leuA at 3088.580 kb on - strand, within leuA at 3088.580 kb on - strand, within leuA
Per-strain Table
Position Strand Gene LocusTag Fraction Lactate-Sulfate (60-30 mM) minimal (MoLS4) remove 3,085,918 - DVU2978 0.73 -1.3 3,085,952 - DVU2978 0.78 +1.1 3,085,952 - DVU2978 0.78 +0.0 3,086,085 - -0.9 3,086,156 - +1.8 3,086,816 + -1.9 3,086,842 - +0.2 3,086,854 - -0.7 3,086,854 - -2.6 3,086,854 - +0.1 3,086,854 - -0.5 3,087,654 + -1.1 3,087,654 + -0.0 3,087,724 + +1.5 3,087,823 + -0.5 3,087,866 + -6.3 3,087,866 + -4.1 3,087,866 + -5.7 3,087,874 - -3.6 3,087,884 - -3.1 3,087,912 + -4.1 3,087,942 + -4.3 3,088,020 - leuA DVU2981 0.12 -3.6 3,088,067 - leuA DVU2981 0.15 -1.3 3,088,088 + leuA DVU2981 0.16 -1.1 3,088,125 + leuA DVU2981 0.19 -1.7 3,088,159 + leuA DVU2981 0.21 -2.4 3,088,203 + leuA DVU2981 0.24 -1.7 3,088,238 + leuA DVU2981 0.26 -2.2 3,088,279 + leuA DVU2981 0.29 -3.5 3,088,324 + leuA DVU2981 0.32 -2.9 3,088,482 + leuA DVU2981 0.42 -3.1 3,088,572 + leuA DVU2981 0.48 -5.0 3,088,580 - leuA DVU2981 0.48 -5.1 3,088,580 - leuA DVU2981 0.48 -1.7
Or see this region's nucleotide sequence