Experiment: growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS11140 and SGL_RS11145 are separated by 359 nucleotides SGL_RS11145 and SGL_RS19910 are separated by 84 nucleotides SGL_RS19910 and SGL_RS11155 are separated by 297 nucleotides SGL_RS11155 and SGL_RS11160 are separated by 159 nucleotides
SGL_RS11140: SGL_RS11140 - citramalate synthase, at 1,959,436 to 1,961,079
_RS11140
SGL_RS11145: SGL_RS11145 - ABC transporter, at 1,961,439 to 1,961,600
_RS11145
SGL_RS19910: SGL_RS19910 - DUF29 domain-containing protein, at 1,961,685 to 1,962,068
_RS19910
SGL_RS11155: SGL_RS11155 - hypothetical protein, at 1,962,366 to 1,962,545
_RS11155
SGL_RS11160: SGL_RS11160 - ParA family protein, at 1,962,705 to 1,965,467
_RS11160
Position (kb)
1961
1962
1963 Strain fitness (log2 ratio)
-1
0
1 at 1960.745 kb on + strand, within SGL_RS11140 at 1960.867 kb on - strand, within SGL_RS11140 at 1961.114 kb on - strand at 1961.224 kb on + strand at 1961.289 kb on - strand at 1961.340 kb on + strand at 1961.838 kb on + strand, within SGL_RS19910 at 1961.879 kb on - strand, within SGL_RS19910 at 1962.005 kb on + strand, within SGL_RS19910 at 1962.013 kb on - strand, within SGL_RS19910 at 1962.083 kb on + strand at 1962.241 kb on + strand at 1962.309 kb on - strand at 1962.880 kb on - strand at 1962.937 kb on - strand at 1962.940 kb on - strand at 1963.052 kb on + strand, within SGL_RS11160
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation remove 1,960,745 + SGL_RS11140 0.80 -0.5 1,960,867 - SGL_RS11140 0.87 -1.4 1,961,114 - -0.2 1,961,224 + +0.3 1,961,289 - +0.3 1,961,340 + -0.8 1,961,838 + SGL_RS19910 0.40 +0.4 1,961,879 - SGL_RS19910 0.51 +0.3 1,962,005 + SGL_RS19910 0.83 -0.6 1,962,013 - SGL_RS19910 0.85 +0.5 1,962,083 + +0.1 1,962,241 + -0.1 1,962,309 - +0.1 1,962,880 - -0.3 1,962,937 - +0.1 1,962,940 - +0.5 1,963,052 + SGL_RS11160 0.13 -0.1
Or see this region's nucleotide sequence