Experiment: growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS00755 and SGL_RS00760 overlap by 17 nucleotides SGL_RS00760 and SGL_RS18535 are separated by 288 nucleotides SGL_RS18535 and SGL_RS00770 are separated by 209 nucleotides SGL_RS00770 and SGL_RS00775 are separated by 29 nucleotides SGL_RS00775 and SGL_RS00780 overlap by 4 nucleotides
SGL_RS00755: SGL_RS00755 - GNAT family N-acetyltransferase, at 28,850 to 29,353
_RS00755
SGL_RS00760: SGL_RS00760 - DUF1778 domain-containing protein, at 29,337 to 29,639
_RS00760
SGL_RS18535: SGL_RS18535 - hypothetical protein, at 29,928 to 30,142
_RS18535
SGL_RS00770: SGL_RS00770 - DUF86 domain-containing protein, at 30,352 to 30,669
_RS00770
SGL_RS00775: SGL_RS00775 - DUF5615 family PIN-like protein, at 30,699 to 31,040
_RS00775
SGL_RS00780: SGL_RS00780 - DUF433 domain-containing protein, at 31,037 to 31,369
_RS00780
Position (kb) 29
30
31 Strain fitness (log2 ratio) -1
0
1 at 29.053 kb on - strand, within SGL_RS00755 at 29.097 kb on - strand, within SGL_RS00755 at 29.097 kb on - strand, within SGL_RS00755 at 29.287 kb on + strand, within SGL_RS00755 at 29.325 kb on - strand at 29.614 kb on + strand at 29.955 kb on + strand, within SGL_RS18535 at 30.063 kb on - strand, within SGL_RS18535 at 30.147 kb on - strand at 30.157 kb on - strand at 30.232 kb on + strand at 30.232 kb on + strand at 30.240 kb on - strand at 30.261 kb on - strand at 30.284 kb on - strand at 30.842 kb on - strand, within SGL_RS00775 at 30.979 kb on + strand, within SGL_RS00775 at 30.991 kb on + strand, within SGL_RS00775 at 31.064 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation remove 29,053 - SGL_RS00755 0.40 +0.1 29,097 - SGL_RS00755 0.49 +0.1 29,097 - SGL_RS00755 0.49 +0.0 29,287 + SGL_RS00755 0.87 +0.4 29,325 - -0.3 29,614 + -0.6 29,955 + SGL_RS18535 0.13 +0.5 30,063 - SGL_RS18535 0.63 +0.6 30,147 - +0.5 30,157 - +0.1 30,232 + +0.4 30,232 + +0.3 30,240 - -0.4 30,261 - +0.0 30,284 - +0.3 30,842 - SGL_RS00775 0.42 +0.2 30,979 + SGL_RS00775 0.82 +0.2 30,991 + SGL_RS00775 0.85 -0.5 31,064 + -0.4
Or see this region's nucleotide sequence