Experiment: growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS00290 and SGL_RS18485 are separated by 301 nucleotides SGL_RS18485 and SGL_RS00295 are separated by 90 nucleotides SGL_RS00295 and SGL_RS00300 overlap by 17 nucleotides SGL_RS00300 and SGL_RS00305 are separated by 73 nucleotides
SGL_RS00285: SGL_RS00285 - hypothetical protein, at 65,331 to 65,786
_RS00285
SGL_RS00290: SGL_RS00290 - transposase, at 65,758 to 66,480
_RS00290
SGL_RS18485: SGL_RS18485 - HNH endonuclease, at 66,782 to 67,072
_RS18485
SGL_RS00295: SGL_RS00295 - hypothetical protein, at 67,163 to 67,378
_RS00295
SGL_RS00300: SGL_RS00300 - ParA family protein, at 67,362 to 67,976
_RS00300
SGL_RS00305: SGL_RS00305 - ATP-binding protein, at 68,050 to 68,574
_RS00305
Position (kb)
66
67
68 Strain fitness (log2 ratio)
-1
0
1 at 65.814 kb on + strand at 65.815 kb on + strand at 65.835 kb on - strand, within SGL_RS00290 at 65.842 kb on - strand, within SGL_RS00290 at 65.844 kb on + strand, within SGL_RS00290 at 65.852 kb on - strand, within SGL_RS00290 at 65.852 kb on - strand, within SGL_RS00290 at 65.852 kb on - strand, within SGL_RS00290 at 65.915 kb on - strand, within SGL_RS00290 at 66.003 kb on + strand, within SGL_RS00290 at 66.049 kb on - strand, within SGL_RS00290 at 66.076 kb on + strand, within SGL_RS00290 at 66.085 kb on - strand, within SGL_RS00290 at 66.145 kb on - strand, within SGL_RS00290 at 66.162 kb on - strand, within SGL_RS00290 at 66.184 kb on - strand, within SGL_RS00290 at 66.205 kb on + strand, within SGL_RS00290 at 66.213 kb on - strand, within SGL_RS00290 at 66.298 kb on - strand, within SGL_RS00290 at 66.329 kb on - strand, within SGL_RS00290 at 66.372 kb on + strand, within SGL_RS00290 at 66.403 kb on + strand, within SGL_RS00290 at 66.403 kb on + strand, within SGL_RS00290 at 66.415 kb on + strand at 66.483 kb on - strand at 66.588 kb on + strand at 66.628 kb on + strand at 66.637 kb on - strand at 66.820 kb on + strand, within SGL_RS18485 at 66.821 kb on - strand, within SGL_RS18485 at 66.985 kb on - strand, within SGL_RS18485 at 67.030 kb on + strand, within SGL_RS18485 at 67.030 kb on + strand, within SGL_RS18485 at 67.035 kb on + strand, within SGL_RS18485 at 67.038 kb on - strand, within SGL_RS18485 at 67.038 kb on - strand, within SGL_RS18485 at 67.112 kb on - strand at 67.144 kb on + strand at 67.216 kb on + strand, within SGL_RS00295 at 67.261 kb on + strand, within SGL_RS00295 at 67.261 kb on + strand, within SGL_RS00295 at 67.269 kb on - strand, within SGL_RS00295 at 67.335 kb on + strand, within SGL_RS00295 at 67.341 kb on + strand, within SGL_RS00295 at 67.343 kb on - strand, within SGL_RS00295 at 67.349 kb on - strand, within SGL_RS00295 at 67.370 kb on - strand at 67.451 kb on - strand, within SGL_RS00300 at 67.456 kb on + strand, within SGL_RS00300 at 67.477 kb on + strand, within SGL_RS00300 at 67.477 kb on + strand, within SGL_RS00300 at 67.477 kb on + strand, within SGL_RS00300 at 67.548 kb on + strand, within SGL_RS00300 at 67.549 kb on - strand, within SGL_RS00300 at 67.573 kb on + strand, within SGL_RS00300 at 67.581 kb on - strand, within SGL_RS00300 at 67.581 kb on - strand, within SGL_RS00300 at 67.591 kb on - strand, within SGL_RS00300 at 67.620 kb on - strand, within SGL_RS00300 at 67.645 kb on - strand, within SGL_RS00300 at 67.658 kb on - strand, within SGL_RS00300 at 67.690 kb on - strand, within SGL_RS00300 at 67.783 kb on + strand, within SGL_RS00300 at 67.857 kb on + strand, within SGL_RS00300 at 67.861 kb on + strand, within SGL_RS00300 at 67.862 kb on + strand, within SGL_RS00300 at 67.947 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation remove 65,814 + -0.1 65,815 + +0.5 65,835 - SGL_RS00290 0.11 +0.1 65,842 - SGL_RS00290 0.12 -0.5 65,844 + SGL_RS00290 0.12 +0.0 65,852 - SGL_RS00290 0.13 -0.6 65,852 - SGL_RS00290 0.13 +0.3 65,852 - SGL_RS00290 0.13 -0.1 65,915 - SGL_RS00290 0.22 +0.1 66,003 + SGL_RS00290 0.34 -0.4 66,049 - SGL_RS00290 0.40 +1.1 66,076 + SGL_RS00290 0.44 +1.1 66,085 - SGL_RS00290 0.45 +0.1 66,145 - SGL_RS00290 0.54 +0.1 66,162 - SGL_RS00290 0.56 -0.9 66,184 - SGL_RS00290 0.59 +0.6 66,205 + SGL_RS00290 0.62 -0.3 66,213 - SGL_RS00290 0.63 -1.0 66,298 - SGL_RS00290 0.75 -0.2 66,329 - SGL_RS00290 0.79 -0.3 66,372 + SGL_RS00290 0.85 +0.1 66,403 + SGL_RS00290 0.89 -0.2 66,403 + SGL_RS00290 0.89 -0.0 66,415 + +0.5 66,483 - -0.2 66,588 + -0.1 66,628 + +0.3 66,637 - +0.1 66,820 + SGL_RS18485 0.13 +1.1 66,821 - SGL_RS18485 0.13 +0.2 66,985 - SGL_RS18485 0.70 -0.3 67,030 + SGL_RS18485 0.85 +1.0 67,030 + SGL_RS18485 0.85 +0.2 67,035 + SGL_RS18485 0.87 +0.1 67,038 - SGL_RS18485 0.88 -0.3 67,038 - SGL_RS18485 0.88 +0.1 67,112 - +0.2 67,144 + +0.6 67,216 + SGL_RS00295 0.25 +0.2 67,261 + SGL_RS00295 0.45 -1.6 67,261 + SGL_RS00295 0.45 -0.8 67,269 - SGL_RS00295 0.49 -0.2 67,335 + SGL_RS00295 0.80 +0.4 67,341 + SGL_RS00295 0.82 -0.7 67,343 - SGL_RS00295 0.83 -0.1 67,349 - SGL_RS00295 0.86 -0.5 67,370 - -0.4 67,451 - SGL_RS00300 0.14 -0.2 67,456 + SGL_RS00300 0.15 -0.3 67,477 + SGL_RS00300 0.19 +0.4 67,477 + SGL_RS00300 0.19 +0.5 67,477 + SGL_RS00300 0.19 -0.3 67,548 + SGL_RS00300 0.30 +0.2 67,549 - SGL_RS00300 0.30 -0.1 67,573 + SGL_RS00300 0.34 -1.2 67,581 - SGL_RS00300 0.36 -0.2 67,581 - SGL_RS00300 0.36 +0.5 67,591 - SGL_RS00300 0.37 -0.4 67,620 - SGL_RS00300 0.42 +0.1 67,645 - SGL_RS00300 0.46 -0.0 67,658 - SGL_RS00300 0.48 -0.3 67,690 - SGL_RS00300 0.53 +0.2 67,783 + SGL_RS00300 0.68 -0.0 67,857 + SGL_RS00300 0.80 +0.4 67,861 + SGL_RS00300 0.81 +0.2 67,862 + SGL_RS00300 0.81 +0.1 67,947 + +0.7
Or see this region's nucleotide sequence