Experiment: growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS16775 and SGL_RS20190 are separated by 30 nucleotides SGL_RS20190 and SGL_RS16780 are separated by 30 nucleotides SGL_RS16780 and SGL_RS16785 are separated by 8 nucleotides SGL_RS16785 and SGL_RS16790 are separated by 210 nucleotides SGL_RS16790 and SGL_RS16795 are separated by 218 nucleotides SGL_RS16795 and SGL_RS16800 are separated by 25 nucleotides
SGL_RS16775: SGL_RS16775 - asparagine--tRNA ligase, at 3,222,476 to 3,223,864
_RS16775
SGL_RS20190: SGL_RS20190 - hypothetical protein, at 3,223,895 to 3,224,026
_RS20190
SGL_RS16780: SGL_RS16780 - tRNA-Tyr, at 3,224,057 to 3,224,138
_RS16780
SGL_RS16785: SGL_RS16785 - tRNA-Thr, at 3,224,147 to 3,224,218
_RS16785
SGL_RS16790: SGL_RS16790 - hypothetical protein, at 3,224,429 to 3,225,409
_RS16790
SGL_RS16795: SGL_RS16795 - TrkA family potassium uptake protein, at 3,225,628 to 3,226,314
_RS16795
SGL_RS16800: SGL_RS16800 - ornithine carbamoyltransferase, at 3,226,340 to 3,227,266
_RS16800
Position (kb)
3224
3225
3226 Strain fitness (log2 ratio)
-1
0
1 at 3223.448 kb on - strand, within SGL_RS16775 at 3223.465 kb on - strand, within SGL_RS16775 at 3223.545 kb on + strand, within SGL_RS16775 at 3223.598 kb on - strand, within SGL_RS16775 at 3223.724 kb on - strand, within SGL_RS16775 at 3223.776 kb on + strand at 3223.785 kb on + strand at 3223.804 kb on + strand at 3223.883 kb on - strand at 3223.913 kb on + strand, within SGL_RS20190 at 3223.957 kb on + strand, within SGL_RS20190 at 3224.258 kb on - strand at 3224.395 kb on + strand at 3224.538 kb on + strand, within SGL_RS16790 at 3224.595 kb on + strand, within SGL_RS16790 at 3224.794 kb on + strand, within SGL_RS16790 at 3224.847 kb on - strand, within SGL_RS16790 at 3226.136 kb on + strand, within SGL_RS16795
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation remove 3,223,448 - SGL_RS16775 0.70 -0.4 3,223,465 - SGL_RS16775 0.71 +0.3 3,223,545 + SGL_RS16775 0.77 -0.4 3,223,598 - SGL_RS16775 0.81 +0.6 3,223,724 - SGL_RS16775 0.90 -0.2 3,223,776 + -0.7 3,223,785 + -0.1 3,223,804 + +0.8 3,223,883 - -0.1 3,223,913 + SGL_RS20190 0.14 -0.6 3,223,957 + SGL_RS20190 0.47 +0.2 3,224,258 - -0.1 3,224,395 + +0.3 3,224,538 + SGL_RS16790 0.11 +0.3 3,224,595 + SGL_RS16790 0.17 +0.8 3,224,794 + SGL_RS16790 0.37 +0.5 3,224,847 - SGL_RS16790 0.43 +0.4 3,226,136 + SGL_RS16795 0.74 +0.6
Or see this region's nucleotide sequence