Experiment: growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14390 and SGL_RS14395 are separated by 116 nucleotides SGL_RS14395 and SGL_RS14400 are separated by 197 nucleotides SGL_RS14400 and SGL_RS14405 are separated by 53 nucleotides SGL_RS14405 and SGL_RS14415 are separated by 402 nucleotides
SGL_RS14390: SGL_RS14390 - histidine phosphatase family protein, at 2,683,443 to 2,684,081
_RS14390
SGL_RS14395: SGL_RS14395 - hypothetical protein, at 2,684,198 to 2,684,824
_RS14395
SGL_RS14400: SGL_RS14400 - cation:proton antiporter, at 2,685,022 to 2,687,055
_RS14400
SGL_RS14405: SGL_RS14405 - DUF29 family protein, at 2,687,109 to 2,687,549
_RS14405
SGL_RS14415: SGL_RS14415 - DNA topoisomerase (ATP-hydrolyzing) subunit A, at 2,687,952 to 2,690,534
_RS14415
Position (kb)
2685
2686
2687
2688 Strain fitness (log2 ratio)
-1
0
1 at 2684.171 kb on + strand at 2684.364 kb on - strand, within SGL_RS14395 at 2684.457 kb on - strand, within SGL_RS14395 at 2684.592 kb on - strand, within SGL_RS14395 at 2684.592 kb on - strand, within SGL_RS14395 at 2684.730 kb on + strand, within SGL_RS14395 at 2684.754 kb on + strand, within SGL_RS14395 at 2684.779 kb on - strand at 2684.783 kb on + strand at 2684.783 kb on + strand at 2684.791 kb on - strand at 2684.791 kb on - strand at 2684.791 kb on - strand at 2684.791 kb on - strand at 2684.796 kb on - strand at 2684.813 kb on - strand at 2684.835 kb on + strand at 2684.852 kb on + strand at 2685.026 kb on + strand at 2685.254 kb on - strand, within SGL_RS14400 at 2685.268 kb on - strand, within SGL_RS14400 at 2685.309 kb on - strand, within SGL_RS14400 at 2685.341 kb on + strand, within SGL_RS14400 at 2685.418 kb on - strand, within SGL_RS14400 at 2685.437 kb on - strand, within SGL_RS14400 at 2685.518 kb on - strand, within SGL_RS14400 at 2685.665 kb on + strand, within SGL_RS14400 at 2685.678 kb on - strand, within SGL_RS14400 at 2685.895 kb on - strand, within SGL_RS14400 at 2685.944 kb on + strand, within SGL_RS14400 at 2685.944 kb on - strand, within SGL_RS14400 at 2685.945 kb on - strand, within SGL_RS14400 at 2685.956 kb on + strand, within SGL_RS14400 at 2686.020 kb on + strand, within SGL_RS14400 at 2686.042 kb on + strand, within SGL_RS14400 at 2686.066 kb on + strand, within SGL_RS14400 at 2686.066 kb on + strand, within SGL_RS14400 at 2686.068 kb on + strand, within SGL_RS14400 at 2686.082 kb on - strand, within SGL_RS14400 at 2686.152 kb on + strand, within SGL_RS14400 at 2686.160 kb on - strand, within SGL_RS14400 at 2686.188 kb on - strand, within SGL_RS14400 at 2686.213 kb on + strand, within SGL_RS14400 at 2686.216 kb on - strand, within SGL_RS14400 at 2686.221 kb on - strand, within SGL_RS14400 at 2686.422 kb on + strand, within SGL_RS14400 at 2686.460 kb on + strand, within SGL_RS14400 at 2686.488 kb on + strand, within SGL_RS14400 at 2686.605 kb on + strand, within SGL_RS14400 at 2686.615 kb on + strand, within SGL_RS14400 at 2686.617 kb on + strand, within SGL_RS14400 at 2686.646 kb on + strand, within SGL_RS14400 at 2686.707 kb on - strand, within SGL_RS14400 at 2686.757 kb on + strand, within SGL_RS14400 at 2686.757 kb on + strand, within SGL_RS14400 at 2686.765 kb on - strand, within SGL_RS14400 at 2686.925 kb on + strand at 2686.981 kb on - strand at 2686.986 kb on - strand at 2687.043 kb on + strand at 2687.389 kb on - strand, within SGL_RS14405 at 2687.415 kb on + strand, within SGL_RS14405 at 2687.694 kb on - strand at 2687.694 kb on - strand at 2687.768 kb on - strand at 2687.822 kb on + strand at 2687.841 kb on - strand at 2687.850 kb on + strand at 2687.850 kb on + strand at 2687.858 kb on + strand at 2687.864 kb on - strand at 2687.918 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation remove 2,684,171 + +0.6 2,684,364 - SGL_RS14395 0.26 -0.2 2,684,457 - SGL_RS14395 0.41 +0.2 2,684,592 - SGL_RS14395 0.63 -0.2 2,684,592 - SGL_RS14395 0.63 +0.3 2,684,730 + SGL_RS14395 0.85 -0.2 2,684,754 + SGL_RS14395 0.89 +0.5 2,684,779 - +0.2 2,684,783 + -0.1 2,684,783 + +1.1 2,684,791 - -0.2 2,684,791 - -0.1 2,684,791 - +0.3 2,684,791 - +0.4 2,684,796 - +0.0 2,684,813 - +0.4 2,684,835 + -0.2 2,684,852 + -0.4 2,685,026 + -0.3 2,685,254 - SGL_RS14400 0.11 +0.3 2,685,268 - SGL_RS14400 0.12 +1.6 2,685,309 - SGL_RS14400 0.14 +0.6 2,685,341 + SGL_RS14400 0.16 +0.5 2,685,418 - SGL_RS14400 0.19 +0.2 2,685,437 - SGL_RS14400 0.20 +0.4 2,685,518 - SGL_RS14400 0.24 +0.1 2,685,665 + SGL_RS14400 0.32 +1.2 2,685,678 - SGL_RS14400 0.32 +0.7 2,685,895 - SGL_RS14400 0.43 +1.1 2,685,944 + SGL_RS14400 0.45 +0.6 2,685,944 - SGL_RS14400 0.45 +0.6 2,685,945 - SGL_RS14400 0.45 -0.2 2,685,956 + SGL_RS14400 0.46 +0.6 2,686,020 + SGL_RS14400 0.49 +0.2 2,686,042 + SGL_RS14400 0.50 -0.2 2,686,066 + SGL_RS14400 0.51 +0.8 2,686,066 + SGL_RS14400 0.51 +0.2 2,686,068 + SGL_RS14400 0.51 +0.5 2,686,082 - SGL_RS14400 0.52 +0.4 2,686,152 + SGL_RS14400 0.56 +0.7 2,686,160 - SGL_RS14400 0.56 -0.6 2,686,188 - SGL_RS14400 0.57 +0.4 2,686,213 + SGL_RS14400 0.59 +0.5 2,686,216 - SGL_RS14400 0.59 +0.1 2,686,221 - SGL_RS14400 0.59 -0.0 2,686,422 + SGL_RS14400 0.69 +0.1 2,686,460 + SGL_RS14400 0.71 +0.1 2,686,488 + SGL_RS14400 0.72 +0.7 2,686,605 + SGL_RS14400 0.78 +0.4 2,686,615 + SGL_RS14400 0.78 +0.2 2,686,617 + SGL_RS14400 0.78 +0.3 2,686,646 + SGL_RS14400 0.80 +0.3 2,686,707 - SGL_RS14400 0.83 +0.6 2,686,757 + SGL_RS14400 0.85 +0.5 2,686,757 + SGL_RS14400 0.85 +0.5 2,686,765 - SGL_RS14400 0.86 -0.1 2,686,925 + +0.1 2,686,981 - -0.1 2,686,986 - -0.2 2,687,043 + +0.5 2,687,389 - SGL_RS14405 0.63 -0.1 2,687,415 + SGL_RS14405 0.69 -0.1 2,687,694 - +0.3 2,687,694 - +0.1 2,687,768 - +0.1 2,687,822 + -0.5 2,687,841 - +0.3 2,687,850 + +0.2 2,687,850 + -0.2 2,687,858 + -0.3 2,687,864 - +0.1 2,687,918 + -0.9
Or see this region's nucleotide sequence