Experiment: growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS13580 and SGL_RS13585 are separated by 136 nucleotides SGL_RS13585 and SGL_RS13590 overlap by 28 nucleotides
SGL_RS13580: SGL_RS13580 - FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO, at 2,516,814 to 2,518,184
_RS13580
SGL_RS13585: SGL_RS13585 - PAS domain S-box protein, at 2,518,321 to 2,521,857
_RS13585
SGL_RS13590: SGL_RS13590 - tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG, at 2,521,830 to 2,523,737
_RS13590
Position (kb)
2518
2519
2520
2521
2522 Strain fitness (log2 ratio)
-1
0
1 at 2517.420 kb on + strand, within SGL_RS13580 at 2517.433 kb on + strand, within SGL_RS13580 at 2517.441 kb on - strand, within SGL_RS13580 at 2517.561 kb on - strand, within SGL_RS13580 at 2517.691 kb on - strand, within SGL_RS13580 at 2517.765 kb on - strand, within SGL_RS13580 at 2517.793 kb on - strand, within SGL_RS13580 at 2517.847 kb on - strand, within SGL_RS13580 at 2517.903 kb on + strand, within SGL_RS13580 at 2517.903 kb on + strand, within SGL_RS13580 at 2517.909 kb on + strand, within SGL_RS13580 at 2518.031 kb on + strand, within SGL_RS13580 at 2518.034 kb on - strand, within SGL_RS13580 at 2518.055 kb on + strand at 2518.140 kb on + strand at 2518.165 kb on - strand at 2518.169 kb on + strand at 2518.177 kb on - strand at 2518.249 kb on - strand at 2518.268 kb on - strand at 2518.310 kb on + strand at 2518.386 kb on + strand at 2518.436 kb on + strand at 2519.359 kb on - strand, within SGL_RS13585 at 2519.409 kb on + strand, within SGL_RS13585 at 2519.419 kb on - strand, within SGL_RS13585 at 2519.948 kb on + strand, within SGL_RS13585 at 2519.948 kb on + strand, within SGL_RS13585 at 2519.951 kb on - strand, within SGL_RS13585 at 2519.956 kb on - strand, within SGL_RS13585 at 2519.956 kb on - strand, within SGL_RS13585 at 2520.072 kb on - strand, within SGL_RS13585 at 2520.296 kb on - strand, within SGL_RS13585 at 2520.531 kb on + strand, within SGL_RS13585 at 2520.539 kb on + strand, within SGL_RS13585 at 2520.573 kb on - strand, within SGL_RS13585 at 2520.943 kb on - strand, within SGL_RS13585 at 2521.082 kb on - strand, within SGL_RS13585 at 2521.470 kb on + strand, within SGL_RS13585 at 2521.553 kb on - strand at 2521.696 kb on + strand at 2521.707 kb on + strand at 2521.791 kb on - strand at 2521.808 kb on - strand at 2522.314 kb on + strand, within SGL_RS13590 at 2522.807 kb on + strand, within SGL_RS13590
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation remove 2,517,420 + SGL_RS13580 0.44 -0.1 2,517,433 + SGL_RS13580 0.45 -0.1 2,517,441 - SGL_RS13580 0.46 +0.3 2,517,561 - SGL_RS13580 0.54 +0.4 2,517,691 - SGL_RS13580 0.64 -0.1 2,517,765 - SGL_RS13580 0.69 -0.5 2,517,793 - SGL_RS13580 0.71 -0.3 2,517,847 - SGL_RS13580 0.75 +0.1 2,517,903 + SGL_RS13580 0.79 -0.0 2,517,903 + SGL_RS13580 0.79 -0.1 2,517,909 + SGL_RS13580 0.80 -0.1 2,518,031 + SGL_RS13580 0.89 +0.7 2,518,034 - SGL_RS13580 0.89 +0.1 2,518,055 + -0.5 2,518,140 + +0.4 2,518,165 - -0.1 2,518,169 + -0.2 2,518,177 - +0.0 2,518,249 - +0.3 2,518,268 - +0.5 2,518,310 + +0.2 2,518,386 + +0.7 2,518,436 + +0.8 2,519,359 - SGL_RS13585 0.29 +0.8 2,519,409 + SGL_RS13585 0.31 +0.3 2,519,419 - SGL_RS13585 0.31 +0.7 2,519,948 + SGL_RS13585 0.46 +0.2 2,519,948 + SGL_RS13585 0.46 +0.2 2,519,951 - SGL_RS13585 0.46 +0.3 2,519,956 - SGL_RS13585 0.46 -0.6 2,519,956 - SGL_RS13585 0.46 +0.3 2,520,072 - SGL_RS13585 0.50 +0.6 2,520,296 - SGL_RS13585 0.56 -1.0 2,520,531 + SGL_RS13585 0.62 +0.4 2,520,539 + SGL_RS13585 0.63 +1.2 2,520,573 - SGL_RS13585 0.64 +0.8 2,520,943 - SGL_RS13585 0.74 +0.5 2,521,082 - SGL_RS13585 0.78 +0.2 2,521,470 + SGL_RS13585 0.89 -0.3 2,521,553 - +0.7 2,521,696 + +0.6 2,521,707 + +0.5 2,521,791 - +1.2 2,521,808 - +0.5 2,522,314 + SGL_RS13590 0.25 -0.9 2,522,807 + SGL_RS13590 0.51 -1.4
Or see this region's nucleotide sequence