Experiment: growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12360 and SGL_RS12365 are separated by 30 nucleotides SGL_RS12365 and SGL_RS12370 are separated by 224 nucleotides SGL_RS12370 and SGL_RS12375 are separated by 21 nucleotides SGL_RS12375 and SGL_RS19925 are separated by 78 nucleotides SGL_RS19925 and SGL_RS18970 overlap by 91 nucleotides SGL_RS18970 and SGL_RS12390 are separated by 10 nucleotides
SGL_RS12360: SGL_RS12360 - DUF3750 domain-containing protein, at 2,233,809 to 2,234,273
_RS12360
SGL_RS12365: SGL_RS12365 - nucleoside-diphosphate kinase, at 2,234,304 to 2,234,753
_RS12365
SGL_RS12370: SGL_RS12370 - tRNA-Ala, at 2,234,978 to 2,235,050
_RS12370
SGL_RS12375: SGL_RS12375 - hypothetical protein, at 2,235,072 to 2,235,482
_RS12375
SGL_RS19925: SGL_RS19925 - IS630 transposase-related protein, at 2,235,561 to 2,235,920
_RS19925
SGL_RS18970: SGL_RS18970 - IS630 family transposase, at 2,235,830 to 2,236,408
_RS18970
SGL_RS12390: SGL_RS12390 - aromatic ring-hydroxylating dioxygenase subunit alpha, at 2,236,419 to 2,237,054
_RS12390
Position (kb)
2235
2236 Strain fitness (log2 ratio)
-2
-1
0
1 at 2234.116 kb on + strand, within SGL_RS12360 at 2234.158 kb on - strand, within SGL_RS12360 at 2234.328 kb on - strand at 2234.365 kb on - strand, within SGL_RS12365 at 2234.411 kb on + strand, within SGL_RS12365 at 2234.540 kb on - strand, within SGL_RS12365 at 2234.867 kb on - strand at 2234.959 kb on + strand at 2235.159 kb on + strand, within SGL_RS12375 at 2236.447 kb on - strand at 2236.462 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation remove 2,234,116 + SGL_RS12360 0.66 +0.2 2,234,158 - SGL_RS12360 0.75 +0.2 2,234,328 - +0.2 2,234,365 - SGL_RS12365 0.14 -1.7 2,234,411 + SGL_RS12365 0.24 -1.7 2,234,540 - SGL_RS12365 0.52 -2.7 2,234,867 - +0.1 2,234,959 + +0.6 2,235,159 + SGL_RS12375 0.21 +0.5 2,236,447 - +0.3 2,236,462 + -0.2
Or see this region's nucleotide sequence