Experiment: growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS10305 and SGL_RS10310 are separated by 59 nucleotides SGL_RS10310 and SGL_RS10315 are separated by 274 nucleotides SGL_RS10315 and SGL_RS10320 are separated by 192 nucleotides
SGL_RS10305: SGL_RS10305 - PIN/TRAM domain-containing protein, at 1,783,855 to 1,784,934
_RS10305
SGL_RS10310: SGL_RS10310 - hypothetical protein, at 1,784,994 to 1,785,560
_RS10310
SGL_RS10315: SGL_RS10315 - hypothetical protein, at 1,785,835 to 1,788,357
_RS10315
SGL_RS10320: SGL_RS10320 - ribosome biogenesis GTPase Der, at 1,788,550 to 1,789,908
_RS10320
Position (kb)
1785
1786
1787
1788
1789 Strain fitness (log2 ratio)
-2
-1
0
1 at 1784.991 kb on + strand at 1784.991 kb on + strand at 1785.162 kb on - strand, within SGL_RS10310 at 1785.291 kb on + strand, within SGL_RS10310 at 1785.319 kb on - strand, within SGL_RS10310 at 1785.356 kb on - strand, within SGL_RS10310 at 1785.671 kb on + strand at 1785.679 kb on - strand at 1785.726 kb on - strand at 1785.763 kb on + strand at 1785.987 kb on - strand at 1785.996 kb on + strand at 1786.137 kb on - strand, within SGL_RS10315 at 1786.299 kb on - strand, within SGL_RS10315 at 1786.326 kb on - strand, within SGL_RS10315 at 1786.437 kb on + strand, within SGL_RS10315 at 1786.438 kb on - strand, within SGL_RS10315 at 1786.559 kb on + strand, within SGL_RS10315 at 1786.632 kb on + strand, within SGL_RS10315 at 1786.640 kb on - strand, within SGL_RS10315 at 1786.678 kb on - strand, within SGL_RS10315 at 1786.689 kb on - strand, within SGL_RS10315 at 1786.933 kb on + strand, within SGL_RS10315 at 1786.968 kb on - strand, within SGL_RS10315 at 1787.001 kb on - strand, within SGL_RS10315 at 1787.647 kb on + strand, within SGL_RS10315 at 1787.675 kb on - strand, within SGL_RS10315 at 1787.776 kb on - strand, within SGL_RS10315 at 1787.847 kb on + strand, within SGL_RS10315 at 1787.871 kb on - strand, within SGL_RS10315 at 1788.071 kb on - strand, within SGL_RS10315 at 1788.071 kb on - strand, within SGL_RS10315 at 1788.144 kb on - strand at 1788.202 kb on + strand at 1788.202 kb on + strand at 1788.289 kb on + strand at 1788.296 kb on + strand at 1789.021 kb on + strand, within SGL_RS10320 at 1789.100 kb on + strand, within SGL_RS10320 at 1789.178 kb on + strand, within SGL_RS10320 at 1789.186 kb on - strand, within SGL_RS10320
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation remove 1,784,991 + -0.7 1,784,991 + -0.0 1,785,162 - SGL_RS10310 0.30 +0.2 1,785,291 + SGL_RS10310 0.52 +0.3 1,785,319 - SGL_RS10310 0.57 +0.3 1,785,356 - SGL_RS10310 0.64 +0.0 1,785,671 + -0.0 1,785,679 - -0.2 1,785,726 - +0.3 1,785,763 + +0.2 1,785,987 - -0.6 1,785,996 + +0.3 1,786,137 - SGL_RS10315 0.12 +0.6 1,786,299 - SGL_RS10315 0.18 +0.5 1,786,326 - SGL_RS10315 0.19 +0.5 1,786,437 + SGL_RS10315 0.24 +0.3 1,786,438 - SGL_RS10315 0.24 +0.2 1,786,559 + SGL_RS10315 0.29 +0.4 1,786,632 + SGL_RS10315 0.32 +0.9 1,786,640 - SGL_RS10315 0.32 +1.1 1,786,678 - SGL_RS10315 0.33 +0.4 1,786,689 - SGL_RS10315 0.34 +0.8 1,786,933 + SGL_RS10315 0.44 +0.7 1,786,968 - SGL_RS10315 0.45 +0.6 1,787,001 - SGL_RS10315 0.46 +0.4 1,787,647 + SGL_RS10315 0.72 +0.4 1,787,675 - SGL_RS10315 0.73 +0.8 1,787,776 - SGL_RS10315 0.77 +0.3 1,787,847 + SGL_RS10315 0.80 +0.9 1,787,871 - SGL_RS10315 0.81 +0.4 1,788,071 - SGL_RS10315 0.89 +0.6 1,788,071 - SGL_RS10315 0.89 +0.9 1,788,144 - +0.5 1,788,202 + -0.7 1,788,202 + +0.5 1,788,289 + +0.7 1,788,296 + +0.8 1,789,021 + SGL_RS10320 0.35 -2.6 1,789,100 + SGL_RS10320 0.40 -1.0 1,789,178 + SGL_RS10320 0.46 -2.4 1,789,186 - SGL_RS10320 0.47 -1.4
Or see this region's nucleotide sequence