Experiment: growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS06715 and SGL_RS06720 are separated by 153 nucleotides SGL_RS06720 and SGL_RS06725 are separated by 359 nucleotides SGL_RS06725 and SGL_RS06730 are separated by 175 nucleotides
SGL_RS06715: SGL_RS06715 - NAD(P)/FAD-dependent oxidoreductase, at 1,038,038 to 1,039,042
_RS06715
SGL_RS06720: SGL_RS06720 - SH3 domain-containing protein, at 1,039,196 to 1,039,732
_RS06720
SGL_RS06725: SGL_RS06725 - hypothetical protein, at 1,040,092 to 1,040,355
_RS06725
SGL_RS06730: SGL_RS06730 - succinate dehydrogenase/fumarate reductase flavoprotein subunit, at 1,040,531 to 1,042,258
_RS06730
Position (kb)
1039
1040 Strain fitness (log2 ratio)
-1
0
1 at 1038.230 kb on - strand, within SGL_RS06715 at 1038.248 kb on + strand, within SGL_RS06715 at 1038.248 kb on + strand, within SGL_RS06715 at 1038.256 kb on - strand, within SGL_RS06715 at 1038.278 kb on + strand, within SGL_RS06715 at 1038.340 kb on + strand, within SGL_RS06715 at 1038.363 kb on + strand, within SGL_RS06715 at 1038.409 kb on + strand, within SGL_RS06715 at 1038.476 kb on + strand, within SGL_RS06715 at 1038.500 kb on - strand, within SGL_RS06715 at 1038.552 kb on - strand, within SGL_RS06715 at 1038.649 kb on - strand, within SGL_RS06715 at 1038.734 kb on - strand, within SGL_RS06715 at 1038.770 kb on + strand, within SGL_RS06715 at 1038.799 kb on + strand, within SGL_RS06715 at 1038.804 kb on + strand, within SGL_RS06715 at 1038.817 kb on + strand, within SGL_RS06715 at 1038.825 kb on - strand, within SGL_RS06715 at 1038.906 kb on - strand, within SGL_RS06715 at 1038.935 kb on - strand, within SGL_RS06715 at 1038.966 kb on - strand at 1038.966 kb on - strand at 1039.365 kb on - strand, within SGL_RS06720 at 1039.632 kb on - strand, within SGL_RS06720 at 1039.644 kb on + strand, within SGL_RS06720 at 1039.691 kb on - strand at 1039.691 kb on - strand at 1039.692 kb on + strand at 1039.858 kb on + strand at 1039.891 kb on + strand at 1039.899 kb on - strand at 1040.147 kb on - strand, within SGL_RS06725 at 1040.185 kb on + strand, within SGL_RS06725 at 1040.218 kb on - strand, within SGL_RS06725 at 1040.248 kb on - strand, within SGL_RS06725 at 1040.473 kb on + strand at 1040.613 kb on + strand at 1040.632 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation remove 1,038,230 - SGL_RS06715 0.19 +0.0 1,038,248 + SGL_RS06715 0.21 -0.4 1,038,248 + SGL_RS06715 0.21 -0.3 1,038,256 - SGL_RS06715 0.22 -0.1 1,038,278 + SGL_RS06715 0.24 -0.2 1,038,340 + SGL_RS06715 0.30 -0.5 1,038,363 + SGL_RS06715 0.32 -0.8 1,038,409 + SGL_RS06715 0.37 +0.1 1,038,476 + SGL_RS06715 0.44 -0.8 1,038,500 - SGL_RS06715 0.46 -0.1 1,038,552 - SGL_RS06715 0.51 +0.5 1,038,649 - SGL_RS06715 0.61 +0.2 1,038,734 - SGL_RS06715 0.69 +0.2 1,038,770 + SGL_RS06715 0.73 -0.2 1,038,799 + SGL_RS06715 0.76 -0.1 1,038,804 + SGL_RS06715 0.76 +0.0 1,038,817 + SGL_RS06715 0.78 +0.1 1,038,825 - SGL_RS06715 0.78 +0.2 1,038,906 - SGL_RS06715 0.86 +0.1 1,038,935 - SGL_RS06715 0.89 -1.3 1,038,966 - +0.3 1,038,966 - -0.1 1,039,365 - SGL_RS06720 0.31 -0.2 1,039,632 - SGL_RS06720 0.81 +0.1 1,039,644 + SGL_RS06720 0.83 +0.6 1,039,691 - +0.2 1,039,691 - +0.0 1,039,692 + +0.1 1,039,858 + +0.8 1,039,891 + -0.1 1,039,899 - -0.3 1,040,147 - SGL_RS06725 0.21 -0.1 1,040,185 + SGL_RS06725 0.35 -0.4 1,040,218 - SGL_RS06725 0.48 -0.1 1,040,248 - SGL_RS06725 0.59 -0.1 1,040,473 + -0.2 1,040,613 + +0.4 1,040,632 + -0.3
Or see this region's nucleotide sequence