Experiment: growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS05405 and SGL_RS05410 are separated by 48 nucleotides SGL_RS05410 and SGL_RS05415 overlap by 50 nucleotides SGL_RS05415 and SGL_RS05420 are separated by 156 nucleotides
SGL_RS05405: SGL_RS05405 - prepilin-type N-terminal cleavage/methylation domain-containing protein, at 756,053 to 757,195
_RS05405
SGL_RS05410: SGL_RS05410 - type II secretion system protein, at 757,244 to 757,720
_RS05410
SGL_RS05415: SGL_RS05415 - prepilin-type N-terminal cleavage/methylation domain-containing protein, at 757,671 to 758,753
_RS05415
SGL_RS05420: SGL_RS05420 - hypothetical protein, at 758,910 to 761,228
_RS05420
Position (kb) 757
758
759 Strain fitness (log2 ratio) -1
0
1
2
3 at 756.727 kb on - strand, within SGL_RS05405 at 756.733 kb on + strand, within SGL_RS05405 at 756.802 kb on + strand, within SGL_RS05405 at 757.022 kb on + strand, within SGL_RS05405 at 757.022 kb on + strand, within SGL_RS05405 at 757.065 kb on - strand, within SGL_RS05405 at 757.387 kb on - strand, within SGL_RS05410 at 757.504 kb on - strand, within SGL_RS05410 at 758.029 kb on + strand, within SGL_RS05415 at 758.328 kb on - strand, within SGL_RS05415 at 758.362 kb on + strand, within SGL_RS05415 at 758.517 kb on + strand, within SGL_RS05415 at 759.129 kb on - strand at 759.129 kb on - strand at 759.177 kb on + strand, within SGL_RS05420 at 759.248 kb on - strand, within SGL_RS05420 at 759.262 kb on + strand, within SGL_RS05420 at 759.274 kb on - strand, within SGL_RS05420 at 759.345 kb on + strand, within SGL_RS05420 at 759.352 kb on + strand, within SGL_RS05420 at 759.534 kb on - strand, within SGL_RS05420
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation remove 756,727 - SGL_RS05405 0.59 -0.2 756,733 + SGL_RS05405 0.59 +0.1 756,802 + SGL_RS05405 0.66 -0.5 757,022 + SGL_RS05405 0.85 +0.0 757,022 + SGL_RS05405 0.85 +0.2 757,065 - SGL_RS05405 0.89 +0.4 757,387 - SGL_RS05410 0.30 +0.3 757,504 - SGL_RS05410 0.55 -0.9 758,029 + SGL_RS05415 0.33 -0.0 758,328 - SGL_RS05415 0.61 +1.0 758,362 + SGL_RS05415 0.64 -0.1 758,517 + SGL_RS05415 0.78 +1.0 759,129 - +0.0 759,129 - -0.2 759,177 + SGL_RS05420 0.12 +0.9 759,248 - SGL_RS05420 0.15 -0.1 759,262 + SGL_RS05420 0.15 +0.3 759,274 - SGL_RS05420 0.16 +3.1 759,345 + SGL_RS05420 0.19 +0.0 759,352 + SGL_RS05420 0.19 +0.7 759,534 - SGL_RS05420 0.27 +0.5
Or see this region's nucleotide sequence