Experiment: growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04720 and SGL_RS19335 are separated by 91 nucleotides SGL_RS19335 and SGL_RS04725 are separated by 19 nucleotides SGL_RS04725 and SGL_RS04730 are separated by 57 nucleotides
SGL_RS04720: SGL_RS04720 - iron uptake porin, at 611,057 to 612,832
_RS04720
SGL_RS19335: SGL_RS19335 - hypothetical protein, at 612,924 to 613,082
_RS19335
SGL_RS04725: SGL_RS04725 - response regulator transcription factor, at 613,102 to 613,743
_RS04725
SGL_RS04730: SGL_RS04730 - N-acetylmuramoyl-L-alanine amidase, at 613,801 to 614,817
_RS04730
Position (kb) 613
614 Strain fitness (log2 ratio) -2
-1
0
1 at 612.141 kb on + strand, within SGL_RS04720 at 612.252 kb on + strand, within SGL_RS04720 at 612.255 kb on - strand, within SGL_RS04720 at 612.260 kb on - strand, within SGL_RS04720 at 612.811 kb on - strand at 612.836 kb on - strand at 613.161 kb on - strand at 613.218 kb on - strand, within SGL_RS04725 at 613.312 kb on + strand, within SGL_RS04725 at 613.559 kb on - strand, within SGL_RS04725 at 613.659 kb on - strand, within SGL_RS04725 at 613.713 kb on - strand at 613.713 kb on - strand at 613.770 kb on + strand at 613.878 kb on - strand at 613.883 kb on - strand at 613.884 kb on + strand at 613.884 kb on + strand at 613.959 kb on + strand, within SGL_RS04730 at 613.970 kb on + strand, within SGL_RS04730 at 614.073 kb on + strand, within SGL_RS04730 at 614.113 kb on + strand, within SGL_RS04730 at 614.145 kb on + strand, within SGL_RS04730 at 614.153 kb on - strand, within SGL_RS04730 at 614.176 kb on - strand, within SGL_RS04730 at 614.216 kb on - strand, within SGL_RS04730 at 614.296 kb on - strand, within SGL_RS04730 at 614.364 kb on + strand, within SGL_RS04730 at 614.377 kb on + strand, within SGL_RS04730 at 614.551 kb on - strand, within SGL_RS04730 at 614.604 kb on - strand, within SGL_RS04730 at 614.623 kb on - strand, within SGL_RS04730 at 614.627 kb on + strand, within SGL_RS04730 at 614.706 kb on + strand, within SGL_RS04730
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation remove 612,141 + SGL_RS04720 0.61 -1.0 612,252 + SGL_RS04720 0.67 -0.4 612,255 - SGL_RS04720 0.67 -1.4 612,260 - SGL_RS04720 0.68 -1.0 612,811 - -2.2 612,836 - +0.1 613,161 - +0.6 613,218 - SGL_RS04725 0.18 +0.8 613,312 + SGL_RS04725 0.33 +0.8 613,559 - SGL_RS04725 0.71 +0.4 613,659 - SGL_RS04725 0.87 +0.6 613,713 - +0.8 613,713 - -0.0 613,770 + +0.0 613,878 - +0.1 613,883 - +0.0 613,884 + +0.1 613,884 + +0.3 613,959 + SGL_RS04730 0.16 +0.7 613,970 + SGL_RS04730 0.17 -0.4 614,073 + SGL_RS04730 0.27 -1.2 614,113 + SGL_RS04730 0.31 +0.0 614,145 + SGL_RS04730 0.34 +0.3 614,153 - SGL_RS04730 0.35 -0.8 614,176 - SGL_RS04730 0.37 -0.1 614,216 - SGL_RS04730 0.41 +0.5 614,296 - SGL_RS04730 0.49 -0.2 614,364 + SGL_RS04730 0.55 -0.1 614,377 + SGL_RS04730 0.57 +0.8 614,551 - SGL_RS04730 0.74 +0.5 614,604 - SGL_RS04730 0.79 +0.3 614,623 - SGL_RS04730 0.81 -0.0 614,627 + SGL_RS04730 0.81 -0.3 614,706 + SGL_RS04730 0.89 -0.5
Or see this region's nucleotide sequence