Experiment: growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04295 and SGL_RS04300 are separated by 75 nucleotides SGL_RS04300 and SGL_RS18690 are separated by 409 nucleotides SGL_RS18690 and SGL_RS18695 are separated by 48 nucleotides
SGL_RS04295: SGL_RS04295 - glycosyltransferase family 2 protein, at 517,027 to 518,211
_RS04295
SGL_RS04300: SGL_RS04300 - PAS domain S-box protein, at 518,287 to 520,272
_RS04300
SGL_RS18690: SGL_RS18690 - DUF4351 domain-containing protein, at 520,682 to 520,840
_RS18690
SGL_RS18695: SGL_RS18695 - transposase, at 520,889 to 521,357
_RS18695
Position (kb) 518
519
520
521 Strain fitness (log2 ratio) -2
-1
0
1 at 517.321 kb on - strand, within SGL_RS04295 at 517.337 kb on + strand, within SGL_RS04295 at 517.337 kb on + strand, within SGL_RS04295 at 517.345 kb on - strand, within SGL_RS04295 at 517.366 kb on + strand, within SGL_RS04295 at 517.440 kb on - strand, within SGL_RS04295 at 517.445 kb on - strand, within SGL_RS04295 at 517.486 kb on - strand, within SGL_RS04295 at 517.612 kb on - strand, within SGL_RS04295 at 517.704 kb on - strand, within SGL_RS04295 at 517.751 kb on + strand, within SGL_RS04295 at 517.751 kb on + strand, within SGL_RS04295 at 517.759 kb on - strand, within SGL_RS04295 at 517.759 kb on - strand, within SGL_RS04295 at 517.777 kb on - strand, within SGL_RS04295 at 517.787 kb on - strand, within SGL_RS04295 at 517.847 kb on + strand, within SGL_RS04295 at 517.865 kb on - strand, within SGL_RS04295 at 517.865 kb on - strand, within SGL_RS04295 at 517.924 kb on + strand, within SGL_RS04295 at 517.950 kb on + strand, within SGL_RS04295 at 517.996 kb on + strand, within SGL_RS04295 at 518.001 kb on - strand, within SGL_RS04295 at 518.016 kb on + strand, within SGL_RS04295 at 518.030 kb on - strand, within SGL_RS04295 at 518.076 kb on - strand, within SGL_RS04295 at 518.134 kb on - strand at 518.173 kb on - strand at 518.191 kb on + strand at 518.199 kb on - strand at 518.361 kb on + strand at 518.421 kb on - strand at 518.427 kb on - strand at 519.034 kb on + strand, within SGL_RS04300 at 519.650 kb on + strand, within SGL_RS04300 at 519.716 kb on - strand, within SGL_RS04300 at 520.019 kb on - strand, within SGL_RS04300 at 520.107 kb on - strand at 520.725 kb on + strand, within SGL_RS18690 at 520.741 kb on - strand, within SGL_RS18690 at 520.851 kb on - strand at 520.987 kb on - strand, within SGL_RS18695 at 521.044 kb on + strand, within SGL_RS18695 at 521.058 kb on + strand, within SGL_RS18695 at 521.093 kb on + strand, within SGL_RS18695 at 521.160 kb on - strand, within SGL_RS18695
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation remove 517,321 - SGL_RS04295 0.25 -0.5 517,337 + SGL_RS04295 0.26 -0.6 517,337 + SGL_RS04295 0.26 -0.3 517,345 - SGL_RS04295 0.27 -0.3 517,366 + SGL_RS04295 0.29 +0.8 517,440 - SGL_RS04295 0.35 -0.0 517,445 - SGL_RS04295 0.35 -0.0 517,486 - SGL_RS04295 0.39 -1.0 517,612 - SGL_RS04295 0.49 +0.3 517,704 - SGL_RS04295 0.57 -2.0 517,751 + SGL_RS04295 0.61 -0.5 517,751 + SGL_RS04295 0.61 -0.0 517,759 - SGL_RS04295 0.62 -0.6 517,759 - SGL_RS04295 0.62 -0.2 517,777 - SGL_RS04295 0.63 +0.1 517,787 - SGL_RS04295 0.64 -0.3 517,847 + SGL_RS04295 0.69 +0.8 517,865 - SGL_RS04295 0.71 +0.1 517,865 - SGL_RS04295 0.71 -0.1 517,924 + SGL_RS04295 0.76 -0.2 517,950 + SGL_RS04295 0.78 -0.3 517,996 + SGL_RS04295 0.82 +1.1 518,001 - SGL_RS04295 0.82 +0.4 518,016 + SGL_RS04295 0.83 -0.2 518,030 - SGL_RS04295 0.85 +0.3 518,076 - SGL_RS04295 0.89 -0.1 518,134 - +0.1 518,173 - +0.7 518,191 + +0.1 518,199 - +0.5 518,361 + -0.8 518,421 - -0.1 518,427 - +0.1 519,034 + SGL_RS04300 0.38 +0.2 519,650 + SGL_RS04300 0.69 +0.7 519,716 - SGL_RS04300 0.72 +0.4 520,019 - SGL_RS04300 0.87 +0.3 520,107 - +0.0 520,725 + SGL_RS18690 0.27 -0.3 520,741 - SGL_RS18690 0.37 +0.1 520,851 - -0.2 520,987 - SGL_RS18695 0.21 +0.3 521,044 + SGL_RS18695 0.33 -0.2 521,058 + SGL_RS18695 0.36 -0.2 521,093 + SGL_RS18695 0.43 +0.4 521,160 - SGL_RS18695 0.58 +0.1
Or see this region's nucleotide sequence