Experiment: growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS19755 and SGL_RS03155 are separated by 40 nucleotides SGL_RS03155 and SGL_RS03160 are separated by 115 nucleotides SGL_RS03160 and SGL_RS03165 are separated by 126 nucleotides SGL_RS03165 and SGL_RS03170 are separated by 235 nucleotides
SGL_RS19755: SGL_RS19755 - hypothetical protein, at 273,522 to 273,761
_RS19755
SGL_RS03155: SGL_RS03155 - HD domain-containing protein, at 273,802 to 274,386
_RS03155
SGL_RS03160: SGL_RS03160 - 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit CofH, at 274,502 to 275,680
_RS03160
SGL_RS03165: SGL_RS03165 - VOC family protein, at 275,807 to 276,193
_RS03165
SGL_RS03170: SGL_RS03170 - hypothetical protein, at 276,429 to 278,342
_RS03170
Position (kb) 274
275
276 Strain fitness (log2 ratio) -1
0
1 at 273.574 kb on - strand, within SGL_RS19755 at 273.598 kb on - strand, within SGL_RS19755 at 273.618 kb on + strand, within SGL_RS19755 at 273.680 kb on + strand, within SGL_RS19755 at 273.680 kb on + strand, within SGL_RS19755 at 273.858 kb on - strand at 273.966 kb on + strand, within SGL_RS03155 at 274.031 kb on - strand, within SGL_RS03155 at 274.031 kb on - strand, within SGL_RS03155 at 274.101 kb on + strand, within SGL_RS03155 at 274.109 kb on - strand, within SGL_RS03155 at 274.248 kb on - strand, within SGL_RS03155 at 274.259 kb on - strand, within SGL_RS03155 at 274.282 kb on - strand, within SGL_RS03155 at 274.313 kb on - strand, within SGL_RS03155 at 274.328 kb on + strand at 274.336 kb on - strand at 274.357 kb on + strand at 274.450 kb on + strand at 274.725 kb on + strand, within SGL_RS03160 at 274.989 kb on + strand, within SGL_RS03160 at 275.040 kb on + strand, within SGL_RS03160 at 275.313 kb on + strand, within SGL_RS03160 at 275.321 kb on - strand, within SGL_RS03160 at 275.357 kb on - strand, within SGL_RS03160 at 275.402 kb on - strand, within SGL_RS03160 at 275.570 kb on - strand at 275.621 kb on + strand at 275.653 kb on + strand at 275.653 kb on + strand at 275.659 kb on + strand at 275.661 kb on - strand at 275.661 kb on - strand at 275.674 kb on + strand at 275.711 kb on + strand at 275.711 kb on + strand at 275.713 kb on + strand at 275.898 kb on + strand, within SGL_RS03165 at 275.925 kb on - strand, within SGL_RS03165 at 275.939 kb on + strand, within SGL_RS03165 at 275.961 kb on - strand, within SGL_RS03165 at 275.973 kb on - strand, within SGL_RS03165 at 275.973 kb on - strand, within SGL_RS03165 at 276.066 kb on + strand, within SGL_RS03165 at 276.075 kb on + strand, within SGL_RS03165 at 276.200 kb on + strand at 276.259 kb on - strand at 276.265 kb on + strand at 276.314 kb on + strand at 276.389 kb on + strand at 276.612 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation remove 273,574 - SGL_RS19755 0.22 -0.4 273,598 - SGL_RS19755 0.32 +0.3 273,618 + SGL_RS19755 0.40 -0.3 273,680 + SGL_RS19755 0.66 -0.1 273,680 + SGL_RS19755 0.66 +0.7 273,858 - +0.1 273,966 + SGL_RS03155 0.28 -0.3 274,031 - SGL_RS03155 0.39 -0.9 274,031 - SGL_RS03155 0.39 -0.3 274,101 + SGL_RS03155 0.51 -0.3 274,109 - SGL_RS03155 0.52 +0.2 274,248 - SGL_RS03155 0.76 -0.0 274,259 - SGL_RS03155 0.78 -0.1 274,282 - SGL_RS03155 0.82 -0.0 274,313 - SGL_RS03155 0.87 +0.1 274,328 + -0.5 274,336 - -0.6 274,357 + -0.5 274,450 + -0.5 274,725 + SGL_RS03160 0.19 +0.2 274,989 + SGL_RS03160 0.41 +0.4 275,040 + SGL_RS03160 0.46 -0.0 275,313 + SGL_RS03160 0.69 +0.2 275,321 - SGL_RS03160 0.69 +0.4 275,357 - SGL_RS03160 0.73 +0.7 275,402 - SGL_RS03160 0.76 +0.4 275,570 - +0.2 275,621 + -0.1 275,653 + +0.0 275,653 + -0.2 275,659 + -0.1 275,661 - +0.4 275,661 - +0.2 275,674 + +0.6 275,711 + +0.5 275,711 + -0.0 275,713 + +0.5 275,898 + SGL_RS03165 0.24 +0.1 275,925 - SGL_RS03165 0.30 -1.0 275,939 + SGL_RS03165 0.34 -0.7 275,961 - SGL_RS03165 0.40 -0.1 275,973 - SGL_RS03165 0.43 +0.4 275,973 - SGL_RS03165 0.43 -0.4 276,066 + SGL_RS03165 0.67 -0.3 276,075 + SGL_RS03165 0.69 -0.1 276,200 + +0.7 276,259 - -0.4 276,265 + +0.0 276,314 + +0.2 276,389 + -0.0 276,612 + -0.1
Or see this region's nucleotide sequence