Experiment: growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS02175 and SGL_RS02180 overlap by 65 nucleotides SGL_RS02180 and SGL_RS02185 are separated by 268 nucleotides SGL_RS02185 and SGL_RS02190 are separated by 209 nucleotides
SGL_RS02175: SGL_RS02175 - UPF0182 family protein, at 70,944 to 74,042
_RS02175
SGL_RS02180: SGL_RS02180 - hypothetical protein, at 73,978 to 74,229
_RS02180
SGL_RS02185: SGL_RS02185 - PepSY domain-containing protein, at 74,498 to 74,785
_RS02185
SGL_RS02190: SGL_RS02190 - A24 family peptidase, at 74,995 to 75,804
_RS02190
Position (kb) 74
75 Strain fitness (log2 ratio) -2
-1
0
1 at 73.528 kb on + strand, within SGL_RS02175 at 73.541 kb on - strand, within SGL_RS02175 at 73.650 kb on + strand, within SGL_RS02175 at 73.685 kb on + strand, within SGL_RS02175 at 73.695 kb on + strand, within SGL_RS02175 at 73.721 kb on - strand, within SGL_RS02175 at 73.748 kb on + strand at 73.787 kb on + strand at 73.795 kb on - strand at 73.830 kb on + strand at 73.954 kb on + strand at 73.967 kb on - strand at 74.038 kb on - strand at 74.074 kb on - strand, within SGL_RS02180 at 74.107 kb on + strand, within SGL_RS02180 at 74.115 kb on - strand, within SGL_RS02180 at 74.115 kb on - strand, within SGL_RS02180 at 74.115 kb on - strand, within SGL_RS02180 at 74.374 kb on + strand at 74.416 kb on - strand at 74.543 kb on - strand, within SGL_RS02185 at 74.550 kb on + strand, within SGL_RS02185 at 74.644 kb on - strand, within SGL_RS02185 at 74.712 kb on + strand, within SGL_RS02185 at 74.799 kb on + strand at 74.949 kb on + strand at 74.960 kb on + strand at 75.037 kb on + strand at 75.149 kb on + strand, within SGL_RS02190 at 75.268 kb on + strand, within SGL_RS02190 at 75.357 kb on - strand, within SGL_RS02190 at 75.375 kb on + strand, within SGL_RS02190 at 75.685 kb on + strand, within SGL_RS02190
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation remove 73,528 + SGL_RS02175 0.83 +1.0 73,541 - SGL_RS02175 0.84 -0.2 73,650 + SGL_RS02175 0.87 -0.3 73,685 + SGL_RS02175 0.88 +0.3 73,695 + SGL_RS02175 0.89 -0.3 73,721 - SGL_RS02175 0.90 -0.6 73,748 + -0.7 73,787 + +0.1 73,795 - +0.2 73,830 + +0.6 73,954 + -0.1 73,967 - +0.1 74,038 - -0.1 74,074 - SGL_RS02180 0.38 +0.4 74,107 + SGL_RS02180 0.51 +0.9 74,115 - SGL_RS02180 0.54 +0.3 74,115 - SGL_RS02180 0.54 -0.1 74,115 - SGL_RS02180 0.54 -0.2 74,374 + +0.1 74,416 - +0.4 74,543 - SGL_RS02185 0.16 -0.4 74,550 + SGL_RS02185 0.18 +0.4 74,644 - SGL_RS02185 0.51 +1.3 74,712 + SGL_RS02185 0.74 +0.3 74,799 + +0.2 74,949 + -0.4 74,960 + -0.0 75,037 + -2.4 75,149 + SGL_RS02190 0.19 -1.7 75,268 + SGL_RS02190 0.34 -1.0 75,357 - SGL_RS02190 0.45 -1.7 75,375 + SGL_RS02190 0.47 +0.6 75,685 + SGL_RS02190 0.85 -1.7
Or see this region's nucleotide sequence