Experiment: growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS01370 and SGL_RS20090 are separated by 45 nucleotides SGL_RS20090 and SGL_RS01375 are separated by 271 nucleotides SGL_RS01375 and SGL_RS19720 are separated by 99 nucleotides SGL_RS19720 and SGL_RS19725 overlap by 31 nucleotides SGL_RS19725 and SGL_RS01390 overlap by 61 nucleotides
SGL_RS01370: SGL_RS01370 - hybrid sensor histidine kinase/response regulator, at 243 to 3,053
_RS01370
SGL_RS20090: SGL_RS20090 - hypothetical protein, at 3,099 to 3,233
_RS20090
SGL_RS01375: SGL_RS01375 - hypothetical protein, at 3,505 to 3,753
_RS01375
SGL_RS19720: SGL_RS19720 - hypothetical protein, at 3,853 to 4,080
_RS19720
SGL_RS19725: SGL_RS19725 - hypothetical protein, at 4,050 to 4,406
_RS19725
SGL_RS01390: SGL_RS01390 - ATP-binding protein, at 4,346 to 5,251
_RS01390
Position (kb)
3
4 Strain fitness (log2 ratio)
-1
0
1 at 2.510 kb on - strand, within SGL_RS01370 at 2.626 kb on + strand, within SGL_RS01370 at 2.643 kb on + strand, within SGL_RS01370 at 2.729 kb on - strand, within SGL_RS01370 at 2.744 kb on + strand, within SGL_RS01370 at 2.875 kb on - strand at 2.978 kb on + strand at 2.984 kb on + strand at 3.006 kb on - strand at 3.042 kb on + strand at 3.051 kb on - strand at 3.199 kb on - strand, within SGL_RS20090 at 3.234 kb on + strand at 3.543 kb on + strand, within SGL_RS01375 at 4.051 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation remove 2,510 - SGL_RS01370 0.81 +0.2 2,626 + SGL_RS01370 0.85 +0.2 2,643 + SGL_RS01370 0.85 +0.2 2,729 - SGL_RS01370 0.88 +0.6 2,744 + SGL_RS01370 0.89 -0.2 2,875 - +0.4 2,978 + +0.3 2,984 + +0.5 3,006 - +0.2 3,042 + +0.5 3,051 - +0.2 3,199 - SGL_RS20090 0.74 -0.7 3,234 + +0.1 3,543 + SGL_RS01375 0.15 +1.2 4,051 - -0.2
Or see this region's nucleotide sequence