Experiment: growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS01315 and SGL_RS19310 are separated by 267 nucleotides SGL_RS19310 and SGL_RS01320 are separated by 320 nucleotides SGL_RS01320 and SGL_RS19395 are separated by 362 nucleotides SGL_RS19395 and SGL_RS18570 are separated by 10 nucleotides
SGL_RS01315: SGL_RS01315 - WD40 repeat domain-containing protein, at 32,698 to 36,411
_RS01315
SGL_RS19310: SGL_RS19310 - hypothetical protein, at 36,679 to 36,978
_RS19310
SGL_RS01320: SGL_RS01320 - HNH endonuclease, at 37,299 to 38,048
_RS01320
SGL_RS19395: SGL_RS19395 - hypothetical protein, at 38,411 to 38,557
_RS19395
SGL_RS18570: SGL_RS18570 - IS630 family transposase, at 38,568 to 39,415
_RS18570
Position (kb) 37
38
39 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 36.354 kb on + strand at 36.355 kb on + strand at 36.356 kb on + strand at 36.420 kb on - strand at 36.420 kb on - strand at 36.449 kb on + strand at 36.620 kb on + strand at 36.628 kb on - strand at 36.629 kb on - strand at 36.637 kb on - strand at 36.678 kb on + strand at 36.824 kb on + strand, within SGL_RS19310 at 36.833 kb on - strand, within SGL_RS19310 at 36.905 kb on - strand, within SGL_RS19310 at 36.905 kb on - strand, within SGL_RS19310 at 36.905 kb on - strand, within SGL_RS19310 at 37.094 kb on - strand at 37.233 kb on + strand at 37.512 kb on + strand, within SGL_RS01320 at 37.661 kb on - strand, within SGL_RS01320 at 37.766 kb on - strand, within SGL_RS01320 at 37.977 kb on - strand at 38.078 kb on + strand at 38.464 kb on + strand, within SGL_RS19395 at 38.486 kb on + strand, within SGL_RS19395 at 38.486 kb on + strand, within SGL_RS19395
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation remove 36,354 + +0.5 36,355 + +0.2 36,356 + +0.1 36,420 - +0.0 36,420 - -0.2 36,449 + -2.8 36,620 + -0.5 36,628 - -0.3 36,629 - +0.7 36,637 - +0.1 36,678 + -1.1 36,824 + SGL_RS19310 0.48 -0.2 36,833 - SGL_RS19310 0.51 -0.7 36,905 - SGL_RS19310 0.75 +0.1 36,905 - SGL_RS19310 0.75 -0.2 36,905 - SGL_RS19310 0.75 +0.2 37,094 - +0.1 37,233 + -0.1 37,512 + SGL_RS01320 0.28 +1.0 37,661 - SGL_RS01320 0.48 +0.4 37,766 - SGL_RS01320 0.62 -0.1 37,977 - -1.6 38,078 + -0.6 38,464 + SGL_RS19395 0.36 -0.1 38,486 + SGL_RS19395 0.51 -0.0 38,486 + SGL_RS19395 0.51 -1.3
Or see this region's nucleotide sequence