Experiment: growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS01205 and SGL_RS01215 are separated by 750 nucleotides SGL_RS01215 and SGL_RS01220 are separated by 513 nucleotides
SGL_RS01205: SGL_RS01205 - ParB/RepB/Spo0J family partition protein, at 10,998 to 11,867
_RS01205
SGL_RS01215: SGL_RS01215 - hypothetical protein, at 12,618 to 13,181
_RS01215
SGL_RS01220: SGL_RS01220 - hypothetical protein, at 13,695 to 15,104
_RS01220
Position (kb) 12
13
14 Strain fitness (log2 ratio) -2
-1
0
1 at 11.731 kb on + strand, within SGL_RS01205 at 11.760 kb on + strand, within SGL_RS01205 at 11.772 kb on - strand, within SGL_RS01205 at 11.824 kb on - strand at 11.847 kb on - strand at 11.916 kb on + strand at 11.997 kb on + strand at 12.005 kb on + strand at 12.060 kb on - strand at 12.124 kb on + strand at 12.128 kb on + strand at 12.234 kb on + strand at 12.239 kb on + strand at 12.243 kb on - strand at 12.294 kb on - strand at 12.325 kb on + strand at 12.338 kb on + strand at 12.414 kb on + strand at 12.414 kb on + strand at 12.463 kb on - strand at 12.465 kb on + strand at 12.500 kb on - strand at 12.571 kb on + strand at 12.579 kb on - strand at 12.579 kb on - strand at 12.579 kb on - strand at 12.585 kb on + strand at 12.624 kb on - strand at 12.624 kb on - strand at 12.624 kb on - strand at 12.685 kb on + strand, within SGL_RS01215 at 12.685 kb on + strand, within SGL_RS01215 at 12.705 kb on - strand, within SGL_RS01215 at 12.739 kb on - strand, within SGL_RS01215 at 12.744 kb on + strand, within SGL_RS01215 at 12.752 kb on - strand, within SGL_RS01215 at 12.897 kb on - strand, within SGL_RS01215 at 12.897 kb on - strand, within SGL_RS01215 at 12.928 kb on + strand, within SGL_RS01215 at 12.948 kb on + strand, within SGL_RS01215 at 12.957 kb on + strand, within SGL_RS01215 at 12.962 kb on + strand, within SGL_RS01215 at 12.993 kb on - strand, within SGL_RS01215 at 13.009 kb on - strand, within SGL_RS01215 at 13.099 kb on - strand, within SGL_RS01215 at 13.101 kb on + strand, within SGL_RS01215 at 13.109 kb on - strand, within SGL_RS01215 at 13.169 kb on + strand at 13.169 kb on + strand at 13.176 kb on - strand at 13.291 kb on - strand at 13.297 kb on - strand at 13.314 kb on + strand at 13.369 kb on + strand at 13.439 kb on - strand at 13.439 kb on - strand at 13.534 kb on + strand at 13.545 kb on + strand at 13.608 kb on - strand at 13.641 kb on + strand at 13.662 kb on + strand at 13.707 kb on + strand at 14.020 kb on + strand, within SGL_RS01220 at 14.131 kb on + strand, within SGL_RS01220 at 14.131 kb on + strand, within SGL_RS01220 at 14.151 kb on + strand, within SGL_RS01220 at 14.161 kb on - strand, within SGL_RS01220 at 14.175 kb on + strand, within SGL_RS01220
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation remove 11,731 + SGL_RS01205 0.84 +1.7 11,760 + SGL_RS01205 0.88 -0.6 11,772 - SGL_RS01205 0.89 -0.5 11,824 - +0.3 11,847 - +0.3 11,916 + -0.0 11,997 + +0.6 12,005 + -0.3 12,060 - +1.1 12,124 + +0.7 12,128 + +1.1 12,234 + -1.3 12,239 + -1.1 12,243 - +0.3 12,294 - -0.7 12,325 + -0.2 12,338 + -0.4 12,414 + -0.2 12,414 + +0.4 12,463 - +0.2 12,465 + +0.6 12,500 - -2.3 12,571 + -0.3 12,579 - -0.9 12,579 - +1.2 12,579 - -0.8 12,585 + -0.3 12,624 - -0.5 12,624 - -0.1 12,624 - +0.3 12,685 + SGL_RS01215 0.12 +0.8 12,685 + SGL_RS01215 0.12 +0.2 12,705 - SGL_RS01215 0.15 +0.2 12,739 - SGL_RS01215 0.21 -0.2 12,744 + SGL_RS01215 0.22 -1.2 12,752 - SGL_RS01215 0.24 -0.2 12,897 - SGL_RS01215 0.49 +1.0 12,897 - SGL_RS01215 0.49 +0.3 12,928 + SGL_RS01215 0.55 +0.9 12,948 + SGL_RS01215 0.59 +0.7 12,957 + SGL_RS01215 0.60 -0.2 12,962 + SGL_RS01215 0.61 +0.5 12,993 - SGL_RS01215 0.66 +0.7 13,009 - SGL_RS01215 0.69 +0.8 13,099 - SGL_RS01215 0.85 -0.1 13,101 + SGL_RS01215 0.86 +0.2 13,109 - SGL_RS01215 0.87 -0.2 13,169 + -0.3 13,169 + +0.5 13,176 - +0.1 13,291 - -0.3 13,297 - +0.3 13,314 + +0.5 13,369 + -0.3 13,439 - +0.4 13,439 - +0.2 13,534 + +0.5 13,545 + +0.1 13,608 - +0.5 13,641 + -0.4 13,662 + +0.0 13,707 + -0.4 14,020 + SGL_RS01220 0.23 -0.5 14,131 + SGL_RS01220 0.31 +0.5 14,131 + SGL_RS01220 0.31 -0.8 14,151 + SGL_RS01220 0.32 +0.7 14,161 - SGL_RS01220 0.33 +0.8 14,175 + SGL_RS01220 0.34 -0.1
Or see this region's nucleotide sequence