Experiment: growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS00800 and SGL_RS00805 are separated by 10 nucleotides SGL_RS00805 and SGL_RS00810 are separated by 117 nucleotides SGL_RS00810 and SGL_RS00815 overlap by 10 nucleotides SGL_RS00815 and SGL_RS00820 are separated by 65 nucleotides SGL_RS00820 and SGL_RS00825 are separated by 32 nucleotides SGL_RS00825 and SGL_RS00830 overlap by 8 nucleotides
SGL_RS00800: SGL_RS00800 - putative addiction module antidote protein, at 35,891 to 36,178
_RS00800
SGL_RS00805: SGL_RS00805 - type II toxin-antitoxin system RelE/ParE family toxin, at 36,189 to 36,479
_RS00805
SGL_RS00810: SGL_RS00810 - AbrB/MazE/SpoVT family DNA-binding domain-containing protein, at 36,597 to 36,848
_RS00810
SGL_RS00815: SGL_RS00815 - type II toxin-antitoxin system PemK/MazF family toxin, at 36,839 to 37,201
_RS00815
SGL_RS00820: SGL_RS00820 - DUF1819 family protein, at 37,267 to 37,521
_RS00820
SGL_RS00825: SGL_RS00825 - hypothetical protein, at 37,554 to 38,138
_RS00825
SGL_RS00830: SGL_RS00830 - hypothetical protein, at 38,131 to 38,712
_RS00830
Position (kb) 36
37
38 Strain fitness (log2 ratio) -2
-1
0
1 at 35.917 kb on + strand at 35.979 kb on + strand, within SGL_RS00800 at 35.979 kb on + strand, within SGL_RS00800 at 36.068 kb on + strand, within SGL_RS00800 at 36.076 kb on - strand, within SGL_RS00800 at 36.186 kb on + strand at 36.273 kb on - strand, within SGL_RS00805 at 36.295 kb on - strand, within SGL_RS00805 at 36.295 kb on - strand, within SGL_RS00805 at 36.338 kb on + strand, within SGL_RS00805 at 36.375 kb on - strand, within SGL_RS00805 at 36.441 kb on - strand, within SGL_RS00805 at 36.441 kb on - strand, within SGL_RS00805 at 36.461 kb on - strand at 36.582 kb on + strand at 36.653 kb on - strand, within SGL_RS00810 at 36.658 kb on - strand, within SGL_RS00810 at 36.801 kb on - strand, within SGL_RS00810 at 36.855 kb on - strand at 36.857 kb on - strand at 36.880 kb on - strand, within SGL_RS00815 at 37.136 kb on + strand, within SGL_RS00815 at 37.171 kb on - strand at 37.202 kb on + strand at 37.229 kb on - strand at 37.241 kb on - strand at 37.331 kb on - strand, within SGL_RS00820 at 37.344 kb on - strand, within SGL_RS00820 at 37.454 kb on + strand, within SGL_RS00820 at 37.472 kb on - strand, within SGL_RS00820 at 37.476 kb on + strand, within SGL_RS00820 at 37.476 kb on + strand, within SGL_RS00820 at 37.581 kb on + strand at 37.690 kb on + strand, within SGL_RS00825 at 37.722 kb on - strand, within SGL_RS00825 at 37.748 kb on + strand, within SGL_RS00825 at 37.751 kb on - strand, within SGL_RS00825 at 37.756 kb on - strand, within SGL_RS00825 at 37.785 kb on - strand, within SGL_RS00825 at 37.785 kb on - strand, within SGL_RS00825 at 37.925 kb on - strand, within SGL_RS00825 at 38.020 kb on - strand, within SGL_RS00825
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation remove 35,917 + -0.4 35,979 + SGL_RS00800 0.31 -0.0 35,979 + SGL_RS00800 0.31 -0.2 36,068 + SGL_RS00800 0.61 -0.8 36,076 - SGL_RS00800 0.64 +1.5 36,186 + -0.5 36,273 - SGL_RS00805 0.29 -0.2 36,295 - SGL_RS00805 0.36 -0.3 36,295 - SGL_RS00805 0.36 +0.1 36,338 + SGL_RS00805 0.51 +0.5 36,375 - SGL_RS00805 0.64 +0.7 36,441 - SGL_RS00805 0.87 -0.5 36,441 - SGL_RS00805 0.87 +0.1 36,461 - +0.8 36,582 + -0.2 36,653 - SGL_RS00810 0.22 -1.0 36,658 - SGL_RS00810 0.24 +0.5 36,801 - SGL_RS00810 0.81 +0.1 36,855 - -0.0 36,857 - -0.1 36,880 - SGL_RS00815 0.11 +0.7 37,136 + SGL_RS00815 0.82 +0.2 37,171 - -0.8 37,202 + -0.3 37,229 - +0.4 37,241 - -0.8 37,331 - SGL_RS00820 0.25 +0.0 37,344 - SGL_RS00820 0.30 -0.2 37,454 + SGL_RS00820 0.73 +0.2 37,472 - SGL_RS00820 0.80 +0.2 37,476 + SGL_RS00820 0.82 +0.0 37,476 + SGL_RS00820 0.82 -0.0 37,581 + -1.5 37,690 + SGL_RS00825 0.23 -1.0 37,722 - SGL_RS00825 0.29 -1.9 37,748 + SGL_RS00825 0.33 -1.7 37,751 - SGL_RS00825 0.34 -0.6 37,756 - SGL_RS00825 0.35 -2.6 37,785 - SGL_RS00825 0.39 -0.0 37,785 - SGL_RS00825 0.39 -0.9 37,925 - SGL_RS00825 0.63 +0.0 38,020 - SGL_RS00825 0.80 -0.6
Or see this region's nucleotide sequence