Strain Fitness in Synechocystis sp000284455 PCC 6803 around SGL_RS00535

Experiment: growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSGL_RS00530 and SGL_RS00535 are separated by 478 nucleotidesSGL_RS00535 and SGL_RS00540 are separated by 43 nucleotidesSGL_RS00540 and SGL_RS00545 are separated by 165 nucleotides SGL_RS00530: SGL_RS00530 - hypothetical protein, at 104,141 to 105,274 _RS00530 SGL_RS00535: SGL_RS00535 - hypothetical protein, at 105,753 to 106,043 _RS00535 SGL_RS00540: SGL_RS00540 - hypothetical protein, at 106,087 to 106,371 _RS00540 SGL_RS00545: SGL_RS00545 - alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB, at 106,537 to 106,950 _RS00545 Position (kb) 105 106 107Strain fitness (log2 ratio) -1 0 1at 105.162 kb on + strandat 105.246 kb on - strandat 105.310 kb on - strandat 105.318 kb on + strandat 105.318 kb on + strandat 105.488 kb on - strandat 105.541 kb on + strandat 105.616 kb on - strandat 105.620 kb on + strandat 105.630 kb on - strandat 105.712 kb on - strandat 105.982 kb on + strand, within SGL_RS00535at 106.036 kb on - strandat 106.045 kb on + strandat 106.053 kb on - strandat 106.178 kb on + strand, within SGL_RS00540at 106.190 kb on + strand, within SGL_RS00540at 106.242 kb on + strand, within SGL_RS00540at 106.348 kb on - strandat 106.682 kb on - strand, within SGL_RS00545at 106.795 kb on + strand, within SGL_RS00545at 106.848 kb on + strand, within SGL_RS00545at 106.913 kb on + strandat 106.915 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation
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105,162 + +0.2
105,246 - +0.2
105,310 - -0.4
105,318 + -0.8
105,318 + -0.2
105,488 - +0.3
105,541 + +1.7
105,616 - +0.1
105,620 + +0.0
105,630 - +0.2
105,712 - -0.1
105,982 + SGL_RS00535 0.79 +0.4
106,036 - +0.2
106,045 + -0.1
106,053 - +0.0
106,178 + SGL_RS00540 0.32 +0.7
106,190 + SGL_RS00540 0.36 +0.4
106,242 + SGL_RS00540 0.54 +0.3
106,348 - +0.3
106,682 - SGL_RS00545 0.35 -0.3
106,795 + SGL_RS00545 0.62 -0.3
106,848 + SGL_RS00545 0.75 -1.2
106,913 + +0.3
106,915 + -0.2

Or see this region's nucleotide sequence