Experiment: growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS00075 and SGL_RS00080 are separated by 35 nucleotides SGL_RS00080 and SGL_RS00085 overlap by 11 nucleotides SGL_RS00085 and SGL_RS00090 overlap by 14 nucleotides SGL_RS00090 and SGL_RS00095 are separated by 117 nucleotides SGL_RS00095 and SGL_RS00100 are separated by 10 nucleotides SGL_RS00100 and SGL_RS00105 are separated by 10 nucleotides
SGL_RS00075: SGL_RS00075 - HNH endonuclease domain-containing protein, at 21,843 to 23,219
_RS00075
SGL_RS00080: SGL_RS00080 - nucleotidyltransferase domain-containing protein, at 23,255 to 23,572
_RS00080
SGL_RS00085: SGL_RS00085 - DUF86 domain-containing protein, at 23,562 to 23,903
_RS00085
SGL_RS00090: SGL_RS00090 - aminotransferase class V-fold PLP-dependent enzyme, at 23,890 to 24,063
_RS00090
SGL_RS00095: SGL_RS00095 - type II toxin-antitoxin system RelE/ParE family toxin, at 24,181 to 24,462
_RS00095
SGL_RS00100: SGL_RS00100 - HigA family addiction module antitoxin, at 24,473 to 24,787
_RS00100
SGL_RS00105: SGL_RS00105 - cysteine desulfurase family protein, at 24,798 to 25,955
_RS00105
Position (kb) 23
24
25 Strain fitness (log2 ratio) -1
0
1 at 23.154 kb on + strand at 23.610 kb on - strand, within SGL_RS00085 at 23.817 kb on - strand, within SGL_RS00085 at 23.875 kb on + strand at 23.932 kb on + strand, within SGL_RS00090 at 23.968 kb on - strand, within SGL_RS00090 at 24.010 kb on - strand, within SGL_RS00090 at 24.314 kb on + strand, within SGL_RS00095 at 24.322 kb on - strand, within SGL_RS00095 at 24.323 kb on - strand, within SGL_RS00095 at 24.407 kb on - strand, within SGL_RS00095 at 24.842 kb on - strand at 24.887 kb on + strand at 24.931 kb on - strand, within SGL_RS00105 at 24.931 kb on - strand, within SGL_RS00105 at 24.938 kb on - strand, within SGL_RS00105 at 25.023 kb on - strand, within SGL_RS00105 at 25.041 kb on + strand, within SGL_RS00105
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with air enriched in 1% CO2 but diluted to prevent light attenuation remove 23,154 + -0.9 23,610 - SGL_RS00085 0.14 -0.1 23,817 - SGL_RS00085 0.75 +0.1 23,875 + -0.2 23,932 + SGL_RS00090 0.24 +0.6 23,968 - SGL_RS00090 0.45 -0.3 24,010 - SGL_RS00090 0.69 +0.8 24,314 + SGL_RS00095 0.47 +0.6 24,322 - SGL_RS00095 0.50 -0.2 24,323 - SGL_RS00095 0.50 +0.1 24,407 - SGL_RS00095 0.80 -0.3 24,842 - -0.3 24,887 + +0.1 24,931 - SGL_RS00105 0.11 -0.0 24,931 - SGL_RS00105 0.11 +0.0 24,938 - SGL_RS00105 0.12 -0.7 25,023 - SGL_RS00105 0.19 +0.2 25,041 + SGL_RS00105 0.21 +0.2
Or see this region's nucleotide sequence