Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS18300 and SGL_RS18305 are separated by 161 nucleotides SGL_RS18305 and SGL_RS18310 are separated by 35 nucleotides SGL_RS18310 and SGL_RS18315 are separated by 44 nucleotides SGL_RS18315 and SGL_RS18320 are separated by 33 nucleotides
SGL_RS18300: SGL_RS18300 - bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase, at 3,553,977 to 3,555,512
_RS18300
SGL_RS18305: SGL_RS18305 - hypothetical protein, at 3,555,674 to 3,556,075
_RS18305
SGL_RS18310: SGL_RS18310 - class I SAM-dependent methyltransferase, at 3,556,111 to 3,557,094
_RS18310
SGL_RS18315: SGL_RS18315 - DUF3067 family protein, at 3,557,139 to 3,557,447
_RS18315
SGL_RS18320: SGL_RS18320 - serine/threonine-protein kinase, at 3,557,481 to 3,559,088
_RS18320
Position (kb)
3556
3557
3558 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4
5 at 3555.122 kb on + strand, within SGL_RS18300 at 3555.209 kb on + strand, within SGL_RS18300 at 3555.230 kb on - strand, within SGL_RS18300 at 3555.353 kb on + strand, within SGL_RS18300 at 3555.649 kb on - strand at 3555.712 kb on + strand at 3555.884 kb on + strand, within SGL_RS18305 at 3555.951 kb on + strand, within SGL_RS18305 at 3556.012 kb on - strand, within SGL_RS18305 at 3556.013 kb on + strand, within SGL_RS18305 at 3556.109 kb on + strand at 3556.284 kb on - strand, within SGL_RS18310 at 3556.396 kb on - strand, within SGL_RS18310 at 3556.464 kb on - strand, within SGL_RS18310 at 3556.505 kb on - strand, within SGL_RS18310 at 3556.615 kb on + strand, within SGL_RS18310 at 3556.715 kb on - strand, within SGL_RS18310 at 3556.716 kb on + strand, within SGL_RS18310 at 3556.760 kb on + strand, within SGL_RS18310 at 3556.763 kb on + strand, within SGL_RS18310 at 3556.811 kb on - strand, within SGL_RS18310 at 3556.821 kb on - strand, within SGL_RS18310 at 3556.833 kb on + strand, within SGL_RS18310 at 3556.833 kb on + strand, within SGL_RS18310 at 3557.000 kb on - strand at 3557.021 kb on - strand at 3557.098 kb on - strand at 3557.131 kb on + strand at 3557.171 kb on + strand, within SGL_RS18315 at 3557.256 kb on - strand, within SGL_RS18315 at 3557.303 kb on + strand, within SGL_RS18315 at 3557.318 kb on + strand, within SGL_RS18315 at 3557.343 kb on - strand, within SGL_RS18315 at 3557.354 kb on - strand, within SGL_RS18315 at 3557.474 kb on - strand at 3557.474 kb on - strand at 3557.484 kb on - strand at 3557.499 kb on + strand at 3557.499 kb on + strand at 3557.612 kb on + strand at 3557.620 kb on - strand at 3557.622 kb on + strand at 3557.625 kb on - strand at 3557.691 kb on - strand, within SGL_RS18320 at 3557.732 kb on - strand, within SGL_RS18320 at 3557.761 kb on - strand, within SGL_RS18320 at 3557.784 kb on - strand, within SGL_RS18320 at 3557.795 kb on - strand, within SGL_RS18320 at 3557.906 kb on + strand, within SGL_RS18320 at 3557.925 kb on + strand, within SGL_RS18320 at 3558.022 kb on + strand, within SGL_RS18320
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 3,555,122 + SGL_RS18300 0.75 +0.8 3,555,209 + SGL_RS18300 0.80 -0.8 3,555,230 - SGL_RS18300 0.82 -1.5 3,555,353 + SGL_RS18300 0.90 -0.8 3,555,649 - +0.7 3,555,712 + +5.1 3,555,884 + SGL_RS18305 0.52 +4.2 3,555,951 + SGL_RS18305 0.69 +4.8 3,556,012 - SGL_RS18305 0.84 +4.9 3,556,013 + SGL_RS18305 0.84 +4.8 3,556,109 + +3.9 3,556,284 - SGL_RS18310 0.18 +1.5 3,556,396 - SGL_RS18310 0.29 +1.9 3,556,464 - SGL_RS18310 0.36 +2.6 3,556,505 - SGL_RS18310 0.40 +1.7 3,556,615 + SGL_RS18310 0.51 +3.8 3,556,715 - SGL_RS18310 0.61 +2.8 3,556,716 + SGL_RS18310 0.61 +4.9 3,556,760 + SGL_RS18310 0.66 +3.5 3,556,763 + SGL_RS18310 0.66 +4.0 3,556,811 - SGL_RS18310 0.71 +3.4 3,556,821 - SGL_RS18310 0.72 +3.8 3,556,833 + SGL_RS18310 0.73 +2.9 3,556,833 + SGL_RS18310 0.73 +2.9 3,557,000 - +3.3 3,557,021 - +3.4 3,557,098 - -3.6 3,557,131 + -2.0 3,557,171 + SGL_RS18315 0.10 -3.8 3,557,256 - SGL_RS18315 0.38 -4.3 3,557,303 + SGL_RS18315 0.53 -4.5 3,557,318 + SGL_RS18315 0.58 -4.6 3,557,343 - SGL_RS18315 0.66 -5.0 3,557,354 - SGL_RS18315 0.70 -1.2 3,557,474 - -5.4 3,557,474 - -4.7 3,557,484 - -4.1 3,557,499 + -3.7 3,557,499 + -2.7 3,557,612 + -2.7 3,557,620 - -3.6 3,557,622 + -1.8 3,557,625 - -3.0 3,557,691 - SGL_RS18320 0.13 -2.8 3,557,732 - SGL_RS18320 0.16 -3.2 3,557,761 - SGL_RS18320 0.17 -4.1 3,557,784 - SGL_RS18320 0.19 -2.9 3,557,795 - SGL_RS18320 0.20 -3.4 3,557,906 + SGL_RS18320 0.26 -3.1 3,557,925 + SGL_RS18320 0.28 -4.2 3,558,022 + SGL_RS18320 0.34 -1.8
Or see this region's nucleotide sequence