Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS18300 and SGL_RS18305 are separated by 161 nucleotides SGL_RS18305 and SGL_RS18310 are separated by 35 nucleotides
SGL_RS18300: SGL_RS18300 - bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase, at 3,553,977 to 3,555,512
_RS18300
SGL_RS18305: SGL_RS18305 - hypothetical protein, at 3,555,674 to 3,556,075
_RS18305
SGL_RS18310: SGL_RS18310 - class I SAM-dependent methyltransferase, at 3,556,111 to 3,557,094
_RS18310
Position (kb)
3555
3556
3557 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 3554.686 kb on - strand, within SGL_RS18300 at 3554.706 kb on + strand, within SGL_RS18300 at 3554.775 kb on - strand, within SGL_RS18300 at 3555.060 kb on - strand, within SGL_RS18300 at 3555.072 kb on - strand, within SGL_RS18300 at 3555.122 kb on + strand, within SGL_RS18300 at 3555.209 kb on + strand, within SGL_RS18300 at 3555.230 kb on - strand, within SGL_RS18300 at 3555.353 kb on + strand, within SGL_RS18300 at 3555.649 kb on - strand at 3555.712 kb on + strand at 3555.884 kb on + strand, within SGL_RS18305 at 3555.951 kb on + strand, within SGL_RS18305 at 3556.012 kb on - strand, within SGL_RS18305 at 3556.013 kb on + strand, within SGL_RS18305 at 3556.109 kb on + strand at 3556.284 kb on - strand, within SGL_RS18310 at 3556.396 kb on - strand, within SGL_RS18310 at 3556.464 kb on - strand, within SGL_RS18310 at 3556.505 kb on - strand, within SGL_RS18310 at 3556.615 kb on + strand, within SGL_RS18310 at 3556.715 kb on - strand, within SGL_RS18310 at 3556.716 kb on + strand, within SGL_RS18310 at 3556.760 kb on + strand, within SGL_RS18310 at 3556.763 kb on + strand, within SGL_RS18310 at 3556.811 kb on - strand, within SGL_RS18310 at 3556.821 kb on - strand, within SGL_RS18310 at 3556.833 kb on + strand, within SGL_RS18310 at 3556.833 kb on + strand, within SGL_RS18310 at 3557.000 kb on - strand at 3557.021 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 3,554,686 - SGL_RS18300 0.46 -2.0 3,554,706 + SGL_RS18300 0.47 +0.8 3,554,775 - SGL_RS18300 0.52 +0.8 3,555,060 - SGL_RS18300 0.71 -0.2 3,555,072 - SGL_RS18300 0.71 -0.2 3,555,122 + SGL_RS18300 0.75 +0.8 3,555,209 + SGL_RS18300 0.80 -0.8 3,555,230 - SGL_RS18300 0.82 -1.5 3,555,353 + SGL_RS18300 0.90 -0.8 3,555,649 - +0.7 3,555,712 + +5.1 3,555,884 + SGL_RS18305 0.52 +4.2 3,555,951 + SGL_RS18305 0.69 +4.8 3,556,012 - SGL_RS18305 0.84 +4.9 3,556,013 + SGL_RS18305 0.84 +4.8 3,556,109 + +3.9 3,556,284 - SGL_RS18310 0.18 +1.5 3,556,396 - SGL_RS18310 0.29 +1.9 3,556,464 - SGL_RS18310 0.36 +2.6 3,556,505 - SGL_RS18310 0.40 +1.7 3,556,615 + SGL_RS18310 0.51 +3.8 3,556,715 - SGL_RS18310 0.61 +2.8 3,556,716 + SGL_RS18310 0.61 +4.9 3,556,760 + SGL_RS18310 0.66 +3.5 3,556,763 + SGL_RS18310 0.66 +4.0 3,556,811 - SGL_RS18310 0.71 +3.4 3,556,821 - SGL_RS18310 0.72 +3.8 3,556,833 + SGL_RS18310 0.73 +2.9 3,556,833 + SGL_RS18310 0.73 +2.9 3,557,000 - +3.3 3,557,021 - +3.4
Or see this region's nucleotide sequence