Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS18155 and SGL_RS18160 are separated by 60 nucleotides SGL_RS18160 and SGL_RS18165 are separated by 176 nucleotides SGL_RS18165 and SGL_RS18170 are separated by 17 nucleotides SGL_RS18170 and SGL_RS18175 are separated by 35 nucleotides
SGL_RS18155: SGL_RS18155 - cytochrome b6-f complex iron-sulfur subunit, at 3,527,045 to 3,527,581
_RS18155
SGL_RS18160: SGL_RS18160 - DUF2214 family protein, at 3,527,642 to 3,528,088
_RS18160
SGL_RS18165: SGL_RS18165 - hypothetical protein, at 3,528,265 to 3,529,305
_RS18165
SGL_RS18170: SGL_RS18170 - PspA/IM30 family protein, at 3,529,323 to 3,529,994
_RS18170
SGL_RS18175: SGL_RS18175 - homocysteine S-methyltransferase family protein, at 3,530,030 to 3,530,971
_RS18175
Position (kb)
3528
3529
3530 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3527.308 kb on - strand, within SGL_RS18155 at 3527.362 kb on - strand, within SGL_RS18155 at 3527.377 kb on - strand, within SGL_RS18155 at 3527.444 kb on + strand, within SGL_RS18155 at 3527.459 kb on + strand, within SGL_RS18155 at 3527.538 kb on - strand at 3527.551 kb on - strand at 3527.560 kb on + strand at 3527.695 kb on + strand, within SGL_RS18160 at 3527.724 kb on - strand, within SGL_RS18160 at 3528.045 kb on + strand at 3528.089 kb on - strand at 3528.195 kb on + strand at 3528.224 kb on + strand at 3528.269 kb on - strand at 3528.316 kb on + strand at 3528.356 kb on + strand at 3528.368 kb on + strand at 3528.376 kb on - strand, within SGL_RS18165 at 3528.398 kb on + strand, within SGL_RS18165 at 3528.398 kb on + strand, within SGL_RS18165 at 3528.499 kb on + strand, within SGL_RS18165 at 3528.671 kb on + strand, within SGL_RS18165 at 3528.671 kb on + strand, within SGL_RS18165 at 3528.719 kb on - strand, within SGL_RS18165 at 3528.820 kb on + strand, within SGL_RS18165 at 3528.823 kb on - strand, within SGL_RS18165 at 3528.885 kb on + strand, within SGL_RS18165 at 3528.970 kb on - strand, within SGL_RS18165 at 3529.046 kb on - strand, within SGL_RS18165 at 3529.055 kb on + strand, within SGL_RS18165 at 3529.062 kb on + strand, within SGL_RS18165 at 3529.065 kb on - strand, within SGL_RS18165 at 3529.074 kb on + strand, within SGL_RS18165 at 3529.283 kb on + strand at 3529.291 kb on - strand at 3529.297 kb on + strand at 3529.313 kb on - strand at 3529.458 kb on + strand, within SGL_RS18170 at 3529.634 kb on + strand, within SGL_RS18170 at 3529.664 kb on + strand, within SGL_RS18170 at 3530.136 kb on + strand, within SGL_RS18175
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 3,527,308 - SGL_RS18155 0.49 -0.4 3,527,362 - SGL_RS18155 0.59 +0.0 3,527,377 - SGL_RS18155 0.62 +0.3 3,527,444 + SGL_RS18155 0.74 -0.1 3,527,459 + SGL_RS18155 0.77 -0.5 3,527,538 - -0.1 3,527,551 - +0.0 3,527,560 + +0.2 3,527,695 + SGL_RS18160 0.12 -1.5 3,527,724 - SGL_RS18160 0.18 +0.6 3,528,045 + -1.3 3,528,089 - +0.5 3,528,195 + +0.6 3,528,224 + +0.5 3,528,269 - +0.3 3,528,316 + +1.2 3,528,356 + +1.3 3,528,368 + +1.0 3,528,376 - SGL_RS18165 0.11 +1.4 3,528,398 + SGL_RS18165 0.13 +1.3 3,528,398 + SGL_RS18165 0.13 +1.2 3,528,499 + SGL_RS18165 0.22 -0.0 3,528,671 + SGL_RS18165 0.39 +0.8 3,528,671 + SGL_RS18165 0.39 +1.2 3,528,719 - SGL_RS18165 0.44 +1.1 3,528,820 + SGL_RS18165 0.53 +1.9 3,528,823 - SGL_RS18165 0.54 +2.5 3,528,885 + SGL_RS18165 0.60 +0.9 3,528,970 - SGL_RS18165 0.68 +1.0 3,529,046 - SGL_RS18165 0.75 +1.7 3,529,055 + SGL_RS18165 0.76 -0.9 3,529,062 + SGL_RS18165 0.77 +1.0 3,529,065 - SGL_RS18165 0.77 +1.1 3,529,074 + SGL_RS18165 0.78 -0.5 3,529,283 + -1.4 3,529,291 - -1.7 3,529,297 + -1.9 3,529,313 - -0.4 3,529,458 + SGL_RS18170 0.20 -3.6 3,529,634 + SGL_RS18170 0.46 -2.3 3,529,664 + SGL_RS18170 0.51 -1.5 3,530,136 + SGL_RS18175 0.11 +0.2
Or see this region's nucleotide sequence