Experiment: growth in full media bubbled with ambient air but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS17620 and SGL_RS17625 are separated by 228 nucleotides SGL_RS17625 and SGL_RS17630 are separated by 38 nucleotides
SGL_RS17620: SGL_RS17620 - 3-isopropylmalate dehydratase large subunit, at 3,406,416 to 3,407,822
_RS17620
SGL_RS17625: SGL_RS17625 - EAL domain-containing protein, at 3,408,051 to 3,409,316
_RS17625
SGL_RS17630: SGL_RS17630 - glucosylglycerol 3-phosphatase, at 3,409,355 to 3,410,623
_RS17630
Position (kb)
3408
3409
3410 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3407.436 kb on - strand, within SGL_RS17620 at 3407.829 kb on + strand at 3407.837 kb on - strand at 3407.837 kb on - strand at 3407.857 kb on + strand at 3407.881 kb on - strand at 3407.898 kb on + strand at 3407.903 kb on + strand at 3407.999 kb on + strand at 3408.160 kb on + strand at 3408.235 kb on + strand, within SGL_RS17625 at 3408.457 kb on - strand, within SGL_RS17625 at 3408.738 kb on - strand, within SGL_RS17625 at 3408.967 kb on - strand, within SGL_RS17625 at 3408.979 kb on - strand, within SGL_RS17625 at 3409.038 kb on + strand, within SGL_RS17625 at 3409.039 kb on + strand, within SGL_RS17625 at 3409.039 kb on + strand, within SGL_RS17625 at 3409.067 kb on - strand, within SGL_RS17625 at 3409.081 kb on - strand, within SGL_RS17625 at 3409.159 kb on - strand, within SGL_RS17625 at 3409.415 kb on - strand at 3409.471 kb on - strand at 3409.580 kb on + strand, within SGL_RS17630 at 3409.642 kb on - strand, within SGL_RS17630 at 3409.643 kb on - strand, within SGL_RS17630 at 3409.643 kb on - strand, within SGL_RS17630 at 3409.650 kb on + strand, within SGL_RS17630 at 3409.657 kb on + strand, within SGL_RS17630 at 3409.710 kb on - strand, within SGL_RS17630 at 3409.846 kb on - strand, within SGL_RS17630 at 3410.064 kb on - strand, within SGL_RS17630 at 3410.077 kb on - strand, within SGL_RS17630 at 3410.155 kb on + strand, within SGL_RS17630 at 3410.165 kb on + strand, within SGL_RS17630 at 3410.169 kb on + strand, within SGL_RS17630 at 3410.266 kb on - strand, within SGL_RS17630
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air but diluted to prevent light attenuation remove 3,407,436 - SGL_RS17620 0.72 +0.8 3,407,829 + +1.2 3,407,837 - +1.4 3,407,837 - +0.4 3,407,857 + -1.8 3,407,881 - +1.6 3,407,898 + +1.9 3,407,903 + +0.4 3,407,999 + +0.3 3,408,160 + +0.6 3,408,235 + SGL_RS17625 0.15 +2.1 3,408,457 - SGL_RS17625 0.32 +0.8 3,408,738 - SGL_RS17625 0.54 +1.5 3,408,967 - SGL_RS17625 0.72 +1.4 3,408,979 - SGL_RS17625 0.73 +1.8 3,409,038 + SGL_RS17625 0.78 +0.4 3,409,039 + SGL_RS17625 0.78 +1.4 3,409,039 + SGL_RS17625 0.78 +1.4 3,409,067 - SGL_RS17625 0.80 +1.0 3,409,081 - SGL_RS17625 0.81 +1.5 3,409,159 - SGL_RS17625 0.88 +1.2 3,409,415 - -0.0 3,409,471 - +0.5 3,409,580 + SGL_RS17630 0.18 -0.2 3,409,642 - SGL_RS17630 0.23 -0.2 3,409,643 - SGL_RS17630 0.23 +0.8 3,409,643 - SGL_RS17630 0.23 -0.1 3,409,650 + SGL_RS17630 0.23 +0.6 3,409,657 + SGL_RS17630 0.24 +1.0 3,409,710 - SGL_RS17630 0.28 -0.2 3,409,846 - SGL_RS17630 0.39 -0.0 3,410,064 - SGL_RS17630 0.56 +0.2 3,410,077 - SGL_RS17630 0.57 +1.3 3,410,155 + SGL_RS17630 0.63 +0.0 3,410,165 + SGL_RS17630 0.64 +0.7 3,410,169 + SGL_RS17630 0.64 +0.3 3,410,266 - SGL_RS17630 0.72 +0.7
Or see this region's nucleotide sequence